Hesperidin and Rosemary extract alleviates apoptosis and alterations of DNA methyltransferase and targeting microRNA in a rat model of diabetic cardiomyopathy | ||||
Benha Veterinary Medical Journal | ||||
Volume 42, Issue 2, September 2022, Page 31-36 PDF (1.07 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bvmj.2022.148089.1544 | ||||
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Authors | ||||
Samy Ali Hussein1; omayma ragab Abo zaid2; Hussein F Abdelmaksoud3; Taheya F Ismael4; Ghada Fathy Al lawaty 3 | ||||
1Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt. | ||||
2Biochemistry veterinary medicine Banha university | ||||
3Biochemistry- veterinary medicine- benha University-Egypt | ||||
4Nutrition &Clinical Nutrition- veterinary medicine- benha University-Egypt | ||||
Abstract | ||||
Diabetic cardiomyopathy (DCM) is a complication of diabetes results in high mortality due to its oxidative stress. The present study was designed to investigate the effect of rosemary extract and Hesperidin on hyperglycemia-induced oxidative stress, inflammation and cardiac injury in experimentally induced DCM in rats. Fifty rats were divided into five groups. Group Ι: rats fed on normal diet. Group Π: rats received a single dose of streptozotocin at a dose of (50 mg /kg body wt.). Group III: Rats treated with insulin 2 U/rat per day. Group IV: rats treated orally with Rosemary extract at a dose of (200 mg/kg body weight/day). Group V: rats treated with Hesperidin orally at a dose of (100 mg/kg body weight/day). Blood samples and cardiac tissue specimen were collected at the end of the experiment. The obtained results showed a marked increase in blood glucose concentration and upregulation in Endothelin-1(ET-1), Hypoxia inducing factor-1 α (HIF-1α), miR-29 and miRNA130b-3p gene expressions with hypermethylation in global DNA methyltransferase (DNMT) in cardiac tissue of DCM-induced group. Treatment with insulin, rosemary extract and hesperidin exhibited a significant decrease in blood glucose concentration and downregulation in Endothelin-1, miR-29 and miRNA130b-3p expressions with hypomethylation in DNMT, while, HIF-1α showed a significant upregulation as compared to DCM non treated group. In conclusion, these results indicated that rosemary extract and hesperidin may have great therapeutic potential in the treatment of DCM. | ||||
Keywords | ||||
Diabetic cardiomyopathy; rosemary extract; hesperidin; Endothelin-1; DNA methyltransferase | ||||
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