Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate
Saber, A., Elsayed, N., El-Abd, M., Hassan, A., Abo Alkasem, M., marzouk, M., Elsayed, H. (2023). Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate. EKB Journal Management System, 66(3), 59-67. doi: 10.21608/ejchem.2022.160832.7007
abdelmohsen Saber; Nabil hussein Elsayed; Mostafa Abd El-Hameed El-Abd; Amira Aly Hassan; Mohamed Ibrahim Abo Alkasem; mohammed sonhi marzouk; hamed Elsayed. "Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate". EKB Journal Management System, 66, 3, 2023, 59-67. doi: 10.21608/ejchem.2022.160832.7007
Saber, A., Elsayed, N., El-Abd, M., Hassan, A., Abo Alkasem, M., marzouk, M., Elsayed, H. (2023). 'Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate', EKB Journal Management System, 66(3), pp. 59-67. doi: 10.21608/ejchem.2022.160832.7007
Saber, A., Elsayed, N., El-Abd, M., Hassan, A., Abo Alkasem, M., marzouk, M., Elsayed, H. Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate. EKB Journal Management System, 2023; 66(3): 59-67. doi: 10.21608/ejchem.2022.160832.7007

Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate

Egyptian Journal of Chemistry
Volume 66, Issue 3, March 2023, Page 59-67  XML PDF (560.34 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.160832.7007
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Authors
abdelmohsen Saberorcid 1; Nabil hussein Elsayed email orcid 2; Mostafa Abd El-Hameed El-Abdorcid 3; Amira Aly Hassanorcid 4; Mohamed Ibrahim Abo Alkasemorcid 5; mohammed sonhi marzoukorcid 6; hamed Elsayedorcid 7
1Chemistry of Natural and Microbial Products Dept., Pharmaceutical and Drug Industries Research Div.National Research Centre, Dokki, Giza, Egypt P.O. Box: 12622
2NRC, cairo, Egypt
3Chemistry of Natural Products, NRC, Giza, Egypt
4Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Institute, National Research Centre, Dokki, Giza, Egypt.
5Chemistry of natural and microbial products, Pharmaceutical research division. National Research Centre, Giza, Egypt
6chemistry of tanning materials, chemical industry division. NRC
7Chemistry of tanning materials and leather technology department, National Research center
Abstract
Six fungal species (T 1-6) and twelve isolates from bacterial fish wastes (BFW (1-7) and white pickled shavings wastes WPSW (1-5), excluded from Egyptian leather industry were examined for its utilization as a source for industrial gelatin and collagen hydrolysate. The conversion of the native/pretreated WPSW was conducted through protease blends in simultaneous processes. All the conducted studies confirmed the superiority and efficiency of the bacterial isolates in 2-d- shaken cultures at 37 ℃ over all the tested fungal species under the proper conditions for proteases, especially collagenases productivity. The three bacterial isolates BFW5, WPSW3 and WPSW5 proved their prevalence as potent collagenase producers, which stimulates good gelatin formation through the controlled enzymatic hydrolysis of the two WPS (native/pretreated) specimens, while at the same conditions, the isolates BFW7, WPSW3 & WPSW5 identified as (Citrobacter freundii) were nominated as potent hydrolysate producers. BFW5 identified as (Bacilus cirus) crude enzyme mixture surpassed all the others and afforded 42.5% lab scale gelatin formation efficiency, while the recorded hydrolysate % was only 5.97%. On the other hand, the bacterial isolate WPSW5 afforded the highest hydrolysate estimate (7.12%).
Keywords
Enzymatic degradation; white pickled bovine pickled; industrial gelatin; collagen hydrolysate
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