Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations
Danial, S., Gamal, F., Saad, N., Hamdy, H., Adel, G., Elnahas, M., Wael, F., Aboelghar, S., Hesham, N., Tarek, M., Wagdy, H. (2023). Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations. EKB Journal Management System, 66(9), 133-143. doi: 10.21608/ejchem.2022.171668.7134
Semirna Danial; Fatma Gamal; Nada Saad; Hala Hamdy; Ghada Adel; Mahmoud Elnahas; Fady Wael; Sohair Aboelghar; Nada Hesham; Mohamed Tarek; Hebatallah A Wagdy. "Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations". EKB Journal Management System, 66, 9, 2023, 133-143. doi: 10.21608/ejchem.2022.171668.7134
Danial, S., Gamal, F., Saad, N., Hamdy, H., Adel, G., Elnahas, M., Wael, F., Aboelghar, S., Hesham, N., Tarek, M., Wagdy, H. (2023). 'Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations', EKB Journal Management System, 66(9), pp. 133-143. doi: 10.21608/ejchem.2022.171668.7134
Danial, S., Gamal, F., Saad, N., Hamdy, H., Adel, G., Elnahas, M., Wael, F., Aboelghar, S., Hesham, N., Tarek, M., Wagdy, H. Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations. EKB Journal Management System, 2023; 66(9): 133-143. doi: 10.21608/ejchem.2022.171668.7134

Simple Green RP-UPLC.MethodForThe Analysis Of Ganciclovir In Its Bulk Form And Pharmaceutical Preparations

Egyptian Journal of Chemistry
Volume 66, Issue 9, September 2023, Page 133-143  XML PDF (1.22 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.171668.7134
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Authors
Semirna Danial1; Fatma Gamal1; Nada Saad1; Hala Hamdy1; Ghada Adel1; Mahmoud Elnahas2; Fady Wael1; Sohair Aboelghar3; Nada Hesham3; Mohamed Tarekorcid 4; Hebatallah A Wagdy email orcid 5
1The Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt
2The Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt.
3Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo, Egypt
4Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo, Egypt.
5Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British university in Egypt (BUE), Cairo, Egypt
Abstract
Abstract

A green simple validated reversed phase ultra -. performance liquid chromatographic method was developed for the analysis of ganciclovir in both bulk and two different dosage.forms. The optimum separation was reached using AcclaimTM RSLC.120 C18…column..(2.1.x.100.mm,.2.2.µm) at 30°C using the mobile phase composed of methanol (60%) and 20 mM phosphate buffer,. pH (4.00) 40%; at 0.5 mL/min. flow rate. The measurement took place at 253 nm using a photodiode array detector. The method was linear at concentration ranged from 20.00 to 300.00 µg/mL. The limit of detection and the limit of quantification were 7.00.µg/mL and 20.00 µg/mL, respectively. The range for the percentage relative standard deviation of intra.-.day was 0.38 to 1.10 %, while for inter-day precisions was 0.56 to 2.08 %, respectively. The method was accurate with percentage recovery ranged from 98.71 to 101.02 % with percentage relative standard…deviation ranged from 0.38 to 1.10 %. The method was favorably applied for determining of ganciclovir in bulk, pharmaceutical tablet and gel preparation. The greenness of the method was assessed by the analytical. eco-scale system and it was found to be eco-friendly.
Keywords
Anti-viral; Ultra-performance liquid chromatography; validation; green chemistry; Valcyte® tablets; Ganvir® eye gel
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