IMPROVEMENT OF PLEUROTLIS FLORIDA MUSHROOM PRODUCTION BY GAMMA IRRADIATION | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 2, Volume 82, Issue 4, December 2004, Page 1509-1518 PDF (2.22 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2004.275222 | ||||
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| Authors | ||||
| NAHED S. YOUSSEF1; AZZA A. M. SHAHIN2; SAFWAT A. EL-HADDAD3 | ||||
| 1Faculty of Home Economic, Al-Azhar University, Nawag, Tanta, Egypt | ||||
| 2National Center for Radiation Research and Technology (NCRRT), Nasr City, Cairo, Egypt | ||||
| 3Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| Abstract | ||||
| Basid:ospores of Pleurotus florida fungus were irradiated in a trial to improve their cultivation capabilities. Spore suspension was irradiated with gamma radiation at dose rates of 0.05, 0.15, 0.25, 0.35, 0.45, 0.55, 0.65, 0.75 and 1.0 kGy. Viable counts of spores decreased as the irradiation dose was increased. Number of survivors was reduced from 1.2 x 107 to 5.5 x 102 CFU/ml, when maximum irradiation dose was used. Different doses of gamma radiation improved radial growth when different sugar sources were used as compared with either non-irradiated culture or tissue culturing of the fungus. Gamma-irradiated culture (IC) showed high yield over both the non-irradiated and tissue cultured fungus and the highest biological efficiency obtained was 139.27 when irradiated at 1.0 kGy. However, Slight effect on yield over the tissue culture was noticed. On the other hand, irradiated culture with 0.25 kGy resulted in increased digestible protein content, which reached 21.363 % as compared with 19.557 and 14.856 % for the non-irradiated and the tissue culture, respectively. | ||||
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