SOME FACTORS AFFECTING THE VALUE OF PHENOL TEST USED IN WHEAT ( TRIT/CUM AEST/VUM L.) IDENTIFICATION | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 15, Volume 82, Issue 4, December 2004, Page 1657-1664 PDF (2.14 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2004.275312 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| AMAL H. SELIM | ||||
| Seed Technology Research section, Field Crops Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| Abstract | ||||
| The use of phenol test for wheat varietal identification should be taken with some caution where differences in grain colouration might not always give reliable comparison. The objective of the present study were to determine the effect of nitrogen and phosphorous fertilizers supplied to the mother plants, seed weight, temperature and duration of grain staining on phenol testing results. Two field experiments were conducted at El-Gemmiza experimental stations during 2001/2002 and 2002/2003 season. Each experiment included five wheat varieties namely Sakha 94, Gemmiza 7, Gemmiza 9, Gemmiza 10 and Giza 170 and three nitrogen and phosphorous fertilizer treatments. Grains harvested from each treatment were undergone to phenol test according to the International Rules for Seed Testing. The results indicated that wheat varieties were responded differently at varying levels of nitrogen and low level of nitrogen (40 kg N/Fed) was associated with relatively light colors, while high levels of nitrogen (92 kg/Fed) and phosphorous (30 kg P2 Os /Fed) levels with dark colors. Grain weight categ&ies might also react differently to phenol test where large grain stained darker than small ones. Furthermore, grains produced in 2002/2003 season gave lighter colouration than those grains produced in 2001/2002 season. Low staining temperature (17-18° C) caused slow grain coloration while high temperature (26-270 C) caused over staining of grains. There was a gradual change in grain color with time and it would be useful to investigate colouration of grains at hour intenfals to maximize the benefit of phenol test to distinguish between wheat varieties. | ||||
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