GENETIC VARIATION BETWEEN MALES AND FEMALES IN NILE PERCH (Lates niloticus) AS REVEALED BY RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY | ||||
| Assiut Journal of Agricultural Sciences | ||||
| Article 4, Volume 37, Issue 4, December 2006, Page 50-60 PDF (705.86 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajas.2006.275338 | ||||
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| Authors | ||||
| Mervat, M. Hashad; N. T. Hamdoon; E. A. Mohamed | ||||
| Department of Genetics, Faculty of Agricultural, Assiut University Assiut-Egypt | ||||
| Abstract | ||||
| Nile perch (Lates niloticus) is one of the most important aquacultural species in Egypt. The goal of this work is to search for sex-specific DNA sequences in the male and female genomes of Nile perch by comparative random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Fifteen 10-mer arbitrary primers were used in RAPD-PCR analysis in this investigation. All the primers were amplified successfully on the genomic DNA from samples of males and females and yielded distinct RAPD pattern. The numbers of fragments amplified per primer varied between 3 (OPO-17) and 13 (OPO-16) and had a size ranged from 215 to 2060 bp. A total of 104 bands were amplified, and out of them, 7 bands (6.73%) were polymorphic while another 97 bands (93.27 %) were monomorphic. Primers OPA-11 and OPO-17 generated female specific bands, while primers OPO-2 and OPO-14 generated male specific bands. The primer OPE-1 generated two specific bands: the first is specific for males and the second is specific for females. The other primers failed to generate any sex- specific bands. According to our knowledge these are the first sex-specific DNA markers isolated from a Nile perch fish species. | ||||
| Keywords | ||||
| Lates niloticus; RAPD; Sex determination; Sex specific DNA markers | ||||
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