Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water
Madbolly, W., F. Abdelall, M., S. Zaki, S., A. Nour El-Din, H., I. Fahd, M., A. Abdallah, S. (2022). Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water. EKB Journal Management System, 39(2), 148-164. doi: 10.21608/jsrs.2022.275794
walaa Nabil Madbolly; Manal F. Abdelall; Sanaa S. Zaki; Hanan A. Nour El-Din; Mona I. Fahd; Soad A. Abdallah. "Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water". EKB Journal Management System, 39, 2, 2022, 148-164. doi: 10.21608/jsrs.2022.275794
Madbolly, W., F. Abdelall, M., S. Zaki, S., A. Nour El-Din, H., I. Fahd, M., A. Abdallah, S. (2022). 'Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water', EKB Journal Management System, 39(2), pp. 148-164. doi: 10.21608/jsrs.2022.275794
Madbolly, W., F. Abdelall, M., S. Zaki, S., A. Nour El-Din, H., I. Fahd, M., A. Abdallah, S. Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water. EKB Journal Management System, 2022; 39(2): 148-164. doi: 10.21608/jsrs.2022.275794

Biodegradation of Organophosphorus Pesticide (Malathion) by Bacillus sp. FYM31 Isolated from Agriculture Drainage Water

Journal of Scientific Research in Science
Volume 39, Issue 2, December 2022, Page 148-164  XML PDF (1.57 MB)
Document Type: Original Article
DOI: 10.21608/jsrs.2022.275794
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Authors
walaa Nabil Madbolly email orcid 1; Manal F. Abdelall2; Sanaa S. Zaki3; Hanan A. Nour El-Din2; Mona I. Fahd3; Soad A. Abdallah3
1Botany department, faculty of women for arts, science and education , Ain shams university
2Microbial Molecular Biology Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt.
3Botany Department, Faculty of Women for Arts, Science and Education, Ain-Shams University, Cairo, Egypt.
Abstract
Organophosphorus pesticides (OP) are used extensively in many arenas including agriculture and industry leading to humans and agroecosystems disorders. Malathion is one of the OP that are used in agriculture to control pest and protect crops. Also, they harm non-target organisms and affect cruelly water sources, air, and soil quality. The present study aimed to isolate and identify a potent bacterial isolate capable of degrading malathion. Bacterial strain that isolated from Al Fayoum governorate, Egypt exhibited high efficiency for malathion biodegradation. Biodegradation process using minimal salt medium (MSM) supplemented with different malathion concentrations indicated that the bacterium was able to degrade and use malathion as a sole carbon source up to 700 mg/l at 37°C.The potent strain that exhibited biodegradation potential was identified as Bacillus sp. FYM31 and deposited into GenBank with the accession number OK325597.  HPLC proved the effectiveness of malathion removal by Bacillus sp. FYM31 after 12 days of incubation to the level of 70.1% malathion (700 mg/l) degradation. Organophosphorus hydrolase (opd) gene was detected in the potent Bacillus sp. FYM31 strain. Due to the widespread usage of malathion in Egypt's agricultural areas, Bacillus sp. FYM31 can help bio-remediate the polluted areas.
Keywords
Organophosphorus biodegradation; Malathion degrading- bacteria; Bacillus sp. Organophosphorus hydrolase; (opd) gene
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