Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes
Abdalla, H., Gamal Fiteha, Y., Abdel-Salam Rashed, M., Mohamed Ali, R., Mohamed Abdel-Salam Hammad, A., Saber Mohamed Bream, A., Magdy, M. (2022). Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes. EKB Journal Management System, 39(2), 179-193. doi: 10.21608/jsrs.2022.275796
Hanan Altaief Abdalla; Yosur Gamal Fiteha; Mohamed Abdel-Salam Rashed; Ramadan Mohamed Ali; Aya Mohamed Abdel-Salam Hammad; Ahmed Saber Mohamed Bream; Mahmoud Magdy. "Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes". EKB Journal Management System, 39, 2, 2022, 179-193. doi: 10.21608/jsrs.2022.275796
Abdalla, H., Gamal Fiteha, Y., Abdel-Salam Rashed, M., Mohamed Ali, R., Mohamed Abdel-Salam Hammad, A., Saber Mohamed Bream, A., Magdy, M. (2022). 'Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes', EKB Journal Management System, 39(2), pp. 179-193. doi: 10.21608/jsrs.2022.275796
Abdalla, H., Gamal Fiteha, Y., Abdel-Salam Rashed, M., Mohamed Ali, R., Mohamed Abdel-Salam Hammad, A., Saber Mohamed Bream, A., Magdy, M. Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes. EKB Journal Management System, 2022; 39(2): 179-193. doi: 10.21608/jsrs.2022.275796

Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes

Journal of Scientific Research in Science
Volume 39, Issue 2, December 2022, Page 179-193  XML PDF (1.59 MB)
Document Type: Original Article
DOI: 10.21608/jsrs.2022.275796
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Authors
Hanan Altaief Abdalla email 1; Yosur Gamal Fiteha2, 3; Mohamed Abdel-Salam Rashed3; Ramadan Mohamed Ali2; Aya Mohamed Abdel-Salam Hammad2; Ahmed Saber Mohamed Bream4; Mahmoud Magdy3
1Zoology Department, Collage of Women, Ain Shams University, Cairo, Egypt.
2Zoology Department, Faculty of Women, Ain Shams University, Cairo, Egypt.
3Genetics Department, Faculty of Agriculture, Ain shams University, Cairo, Egypt.
4Entomology Dept., Faculty of Sciences, Al-Azhar University, Cairo, Egypt.
Abstract
DNA barcoding is a molecular genetics technique commonly applied for species identification based on the cytochrome c oxidase subunit I (COI) gene located on the mitochondrial DNA genome. Based on our analysis, the DNA barcodes were generated from 16 specimen of Coccinella (family Coccinellidae). All the samples were collected from diverse sites in Egypt and Libya. Morphological traits based on the number of dorsal spots, DNA extraction, PCR amplification of COI, sequencing, nucleotide BLAST, and phylogenetics analyses were used to classify the specimens. A phylogenetic tree was constructed using the maximum likelihood method, including 46 COI sequences (the study and NCBI sequences). Three Coccinella clusters were defined and classified as Coccinella septempunctata, Coccinella novemnotata, and Coccinella undecimpunctata each species clustered into a unique branch. DNA barcodes discriminated clearly against the analyzed species. This study validated the COI efficiency as a marker for DNA barcoding of insects (Coccinella, family Coccinellidae).
Keywords
Coccinella beetles; COI gene; molecular genetics; species ID; phylogeny
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