LasR and NdvB genes of Pseudomonas aeruginosa posed resistance against different antibiotic concentration and develop anti-biofilm properties against certain disinfectants level. | ||||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||||
| Article 17, Volume 3, Issue 1, January 2023, Page 180-196 PDF (1.04 MB) | ||||
| Document Type: Original research articles | ||||
| DOI: 10.21608/aijpms.2022.134314.1131 | ||||
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| Authors | ||||
Youmna T Noaman  1; Lina Jamil1; Sahar Radwan2
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| 1Department of Microbiology and Immunology, Faculty of Pharmacy, Sixth of October University, Cairo, Egypt. | ||||
| 2Department of Microbiology and Immunology, Faculty of pharmacy, Girls, Al-Azhar University, Cairo, Egypt. | ||||
| Abstract | ||||
| Antibiotic resistance has become one of the most common problems that threaten the world and increases the mortality rate. Pseudomonas aeruginosa is reported to be one of commonest Multi-Drug resistant (MDR), which is responsible for 10 to 15% of nosocomial infection worldwide and high death rates ranging from 18 to 61%. We investigate the anti-biofilm activity of different disinfectants on MDR Pseudomonas aeruginosa. Two hundred two water environmental isolates were collected from different hospitals in Cairo, Egypt. Microbact™ Gram-negative system used for identification. That is showed 41.3% of isolates were Pseudomonas aeruginosa. Antibiotic susceptibility test revealed that 34.7% of the isolates were MDR. Biofilm production was determined by Congo red assay (CRA) and Microtitre plate (MTP) method. CRA showed 89% as biofilm producers. MTP method showed 87% were biofilm-forming. MIC of Carbapenems was determined by the broth macrodilution method. It showed that 50% of isolates were resistant. (MIC) Minimum Inhibitory Concentration of 7 disinfectants against 10-selected MDR strong biofilm Pseudomonas isolates was determined using the broth microdilution method. It showed that the most effective disinfectants with the lowest MICs were the Sodium hypochlorite 5% and Povidone-Iodine 10%. The Real-time PCR was done on lasR and ndvb genes for the selected isolate (E20) before and after exposure to both effective disinfectants. The sample E20 showed a significant down regulation for lasR and ndvb genes with both effective disinfectants. Our study showed that Povidone-iodine 10 % at appropriate concentrations at less than 30 minutes has significant anti-biofilm activity against MDR Pseudomonas aeruginosa. | ||||
| Keywords | ||||
| Pseudomonas aeruginosa; biofilm; antibiotic sensitivity; Sodium hypochlorite 5%; Povidone-iodine 10 %; contact time; RT-PCR | ||||
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