First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt
Abdel-Salam, A., Shanan, N., Soliman, D., Ahmed, M. (2022). First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt. EKB Journal Management System, 14(2), 381-388. doi: 10.21608/eajbsc.2022.276644
Aly M. Abdel-Salam; Nermeen Taha Shanan; Doaa Z. Soliman; M. A. Ahmed. "First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt". EKB Journal Management System, 14, 2, 2022, 381-388. doi: 10.21608/eajbsc.2022.276644
Abdel-Salam, A., Shanan, N., Soliman, D., Ahmed, M. (2022). 'First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt', EKB Journal Management System, 14(2), pp. 381-388. doi: 10.21608/eajbsc.2022.276644
Abdel-Salam, A., Shanan, N., Soliman, D., Ahmed, M. First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt. EKB Journal Management System, 2022; 14(2): 381-388. doi: 10.21608/eajbsc.2022.276644

First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 14, Issue 2, December 2022, Page 381-388  XML PDF (657.3 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2022.276644
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Authors
Aly M. Abdel-Salam1; Nermeen Taha Shanan2; Doaa Z. Soliman3; M. A. Ahmed3
1Plant Pathology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt
2Horticulture Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
3Plant Pathology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Abstract
During the inspection of imported grown Lily (Lilium spp.) plants (Acapulco cultivar), symptoms of  Candidatus Phytoplasma asteris (Ca. P. astris) infestation was observed in the experimental fields of the Faculty of Agriculture, Cairo University, Giza governorate. The observed disease symptoms included stunting, scorched and leaf discoloration, flowering bud failure, and reduced quality of bulbs. Nested polymerase chain reaction (PCR) was the key technique employed in this study. Four pairs of primers were used in PCR analysis. In the first amplification, a universal primer pair P1/P7 was used to amplify a 1.8 kbp DNA fragment of the rRNA operon.  In the second nested amplification, the universal primer pairs R16F2n/R16R2 (F2n/R2) and R16F0/R16R1 (F0/R1) and the specific P1/Ayint were used to detect 1.2, 1.44, and 1.5 kbp, respectively, from aster yellows group phytoplasma infecting plants. Containment of phytoplasma infestation in lily was obtained through a periodical spray of infected lily plants with tetracycline hydrochloride antibiotic. Lily plants are vegetatively propagated. This allows the spread of this phytoplasma to several economic crops.
Keywords
Lily; Aster yellows phytoplasma; nested PCR; tetracycline hydrochloride
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