Determination of Fractional Inhibitory Concentration (FIC) Index as A Measure of Synergy of Antibiotics in E. coli O157:H7
Abdulabbas, S., Ibrahim, Z., Muhammed, H., AL-Khazali, N., Ahmed, S., Ai- Tuma, A., Mahdi, F. (2022). Determination of Fractional Inhibitory Concentration (FIC) Index as A Measure of Synergy of Antibiotics in E. coli O157:H7. EKB Journal Management System, 89(2), 7571-7575. doi: 10.21608/ejhm.2022.276689
Sarah Sameer Abdulabbas; Zainab Khaleel Ibrahim; Hayder Ali Muhammed; Noor Ali Yazi AL-Khazali; Saja Talib Ahmed; Amir Fadhil Ai- Tuma; Fatima Mohammed Saeed Mahdi. "Determination of Fractional Inhibitory Concentration (FIC) Index as A Measure of Synergy of Antibiotics in E. coli O157:H7". EKB Journal Management System, 89, 2, 2022, 7571-7575. doi: 10.21608/ejhm.2022.276689
Abdulabbas, S., Ibrahim, Z., Muhammed, H., AL-Khazali, N., Ahmed, S., Ai- Tuma, A., Mahdi, F. (2022). 'Determination of Fractional Inhibitory Concentration (FIC) Index as A Measure of Synergy of Antibiotics in E. coli O157:H7', EKB Journal Management System, 89(2), pp. 7571-7575. doi: 10.21608/ejhm.2022.276689
Abdulabbas, S., Ibrahim, Z., Muhammed, H., AL-Khazali, N., Ahmed, S., Ai- Tuma, A., Mahdi, F. Determination of Fractional Inhibitory Concentration (FIC) Index as A Measure of Synergy of Antibiotics in E. coli O157:H7. EKB Journal Management System, 2022; 89(2): 7571-7575. doi: 10.21608/ejhm.2022.276689

Determination of Fractional Inhibitory Concentration (FIC) Index as A Measure of Synergy of Antibiotics in E. coli O157:H7

The Egyptian Journal of Hospital Medicine
Article 219, Volume 89, Issue 2, October 2022, Page 7571-7575  XML PDF (569.51 K)
DOI: 10.21608/ejhm.2022.276689
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Authors
Sarah Sameer Abdulabbas1; Zainab Khaleel Ibrahim* 2; Hayder Ali Muhammed3; Noor Ali Yazi AL-Khazali1; Saja Talib Ahmed1; Amir Fadhil Ai- Tuma4; Fatima Mohammed Saeed Mahdi1
1College of Pharmacy, University of Al-Zahraa for Women
2College of Medical and Health Techniques, University of Alkafeel, Iraq
3College of Veterinary Medicine, University of Kerbala. Iraq
4College of Medical and Health Technologies, University of Ahlalbayt,Iraq
Abstract
Background: MIC is the lowest concentration of an antibiotic required to inhibit the growth of an organism.
Methods: MIC was done against six conventional antibiotics e.g., Amoxicillin, Ceftriaxone, Gentamycin, Tetracycline, Ciprofloxacin, and Levofloxacin. E. coli O157:H7 was collected from hospital. The antibacterial activity of six conventional antibiotics was assessed against E. coli O157:H7by using the broth microdilution method then the fractional inhibitory concentration (FIC) index was used to define the interactions between antibiotics.
Results: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of antibiotics against E. coli O157:H7 showed that the highest concentration appeared in ciprofloxacin was 62.5 µg/ml. while ceftriaxone and gentamycin were 15.62 µg/ml. amoxicillin and levofloxacin was 7.812 µg/ml. the lowest concentration appeared in Tetracycline was 0.976 µg/ml. The higher FICI was seen in Ceftriaxone + Levofloxacin combination 0.3 % followed by Levofloxacin + Ciprofloxacin (0.1). Conclusions: The antibacterial activity of both Ceftriaxone and Levofloxacin was enhanced by the combination which proved a highly synergistic effect against E. coli O157:H7.
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