In Vivo Investigation of the Ameliorating Effect of Copper Albumin Complex on chondroitin sulfate in Monosodium iodoacetate -Induced Knee Osteoarthritis

Elnakip, Doaa A.; Mostafa, Nashwa A.M.; Metwally, Asmaa Abdelsalam; El-Mahdy, Reham I.; Nassar, Ahmed Y.; Shanab, Obeid; Ghareeb, Khaled; Abdeen, Ahmed; Maher, Zainab M.; Mahdy, Mohamed A. A.; Metwally, Aya Sh.; Salah, Mohammed

doi:10.21608/svu.2022.153878.1219

	In Vivo Investigation of the Ameliorating Effect of Copper Albumin Complex on chondroitin sulfate in Monosodium iodoacetate -Induced Knee Osteoarthritis
SVU-International Journal of Veterinary Sciences
Volume 5, Issue 4 - Serial Number 14, December 2022, Page 153-164 PDF (344.38 K)
Document Type: Research article
DOI: 10.21608/svu.2022.153878.1219
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Authors
Doaa A. Elnakip¹; Nashwa A.M. Mostafa²; Asmaa Abdelsalam Metwally ³; Reham I. El-Mahdy^* ⁴; Ahmed Y. Nassar^* ⁴; Obeid Shanab ¹; Khaled Ghareeb ⁵; Ahmed Abdeen^* ⁶; Zainab M. Maher^* ⁷; Mohamed A. A. Mahdy^* ⁸; Aya Sh. Metwally^* ⁹; Mohammed Salah¹
¹Department of Biochemistry, Faculty of Veterinary Medicine, South Valley University, Qena 83523
²Department of histology and cell biology, Faculty of Medicine, Assiut University, Assiut
³Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528
⁴Department of Medical Biochemistry and Molecular biology, Faculty of Medicine, Assiut University
⁵Department of Animal and poultry behavior and management, Faculty of Veterinary Medicine, South Valley University, Qena 83523
⁶Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh
⁷Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena 83523
⁸Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, Qena 83523
⁹Department of Pharmacology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
Abstract
Osteoarthritis (OA) is a condition that manifests as cartilage deterioration and subchondral bone sclerosis in the joint tissues. The weight-bearing joint is most severely impacted by OA. According to some research, consuming foods high in copper albumin complex (cu-albumin complex) can help with OA-related joint degeneration and pain relief. The current study's objective to determine how oral administration of the cu-albumin complex as an anti-inflammatory medication affected the development of rat knee osteoarthritis (KOA). Fifty adult albino rats were divided into three groups: negative control untreated (n= 10, no KOA induction); positive untreated control (n= 20, KOA induction); and treated group (n= 20, KOA induction with administration of cu-albumin complex). According to the severity of the clinical symptoms, treated and untreated arthritic groups were equally divided into mild and severe groups (n=10). Monosodium iodoacetate (MIA) was used as intra-articular injection for osteoarthritis induction. Rats were euthanized after a month of the beginning of the experiment, and the joints were examined histopathologically and immunohistochemically. It was indicated that the treatment was effective in reducing KOA severity and in improvement of chondroitin sulfate of the affected cartilages. In conclusion, the structure of the chondroitin sulfate in the knee joint cartilages of KOA-affected rats was modified by the cu-albumin complex.
Keywords
Chondroitin sulfate; Copper albumen complex; Immunohistochemistry; Mankin scores; Osteoarthritis


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