CD14 promotor polymorphisms associated with different allergic phenotypes and modulated with house dust mite allergy | ||||
Parasitologists United Journal | ||||
Article 5, Volume 12, Issue 1, April 2019, Page 26-33 PDF (385.45 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/puj.2019.7917.1031 | ||||
View on SCiNiTO | ||||
Authors | ||||
Wafaa Zaki 1, 2; Hedayat Salem3; Hoda Mohammed Elazizy4; Maryam Ebido Ajeeby5 | ||||
1Medical Parasitology department, Faculty of medicine, Suez Canal University, Ismalia, Egypt.+ | ||||
2Departments of Medical laboratory Technology. | ||||
3Clinical pathology,faculty of medicine,Cairo university, Cairo, Egypt and Medical Biology. | ||||
4Medical Biochemistry, Faculty of Medicine, Cairo University, Egypt. | ||||
5Faculty of medicine, Jazan university, Jazan,KSA. | ||||
Abstract | ||||
Background: House dust mite (HDM) constitutes a major cause of allergic disease all over the world; meanwhile interaction between genetic control, environmental factors in the context of allergen exposure may affect allergic phenotype. Cluster of Differentiation 14 (CD14) polymorphisms play a major role in genetic control of allergic phenotype. Objective: We aimed to assess the role of CD14 genetic polymorphisms at two loci A(-1,145)G, and G(-1,359)T in expression of atopic asthma and allergic rhinitis in the context of HDM exposure in Jazan, KSA. Subjects and Methods: Through a case control study, 160 subjects served as 60 atopic asthmatic patients, 40 allergic rhinitis patients and 60 healthy non-allergic controls. Clinical and immunological parameters for the studied subjects were assessed. Then, genotyping of two single nucleotide polymorphisms (SNPs) at A(-1,145)G, and G(-1,359)T, in the promoter region of the CD14 gene was conducted using restriction fragment length polymorphisms (RFLP-PCR). Results: The present study showed that in HDM sensitive subjects there was a significant association between GG genotype variant at A(-1,145)G with atopic asthma patients and another significant association between TT genotype variant at G(-1,359)T with allergic rhinitis patients. Conclusion: The impact of allergy induced by HDMs may be enhanced in individuals with specific CD14 gene variants resulting in exaggerated allergic phenotype. | ||||
Keywords | ||||
atopic asthma; Allergic Rhinitis; CD14 gene polymorphism; house dust mites | ||||
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