Neutrophil Gelatinase-associated Lipocalin-2 and Macrophage antigen -1 in Cirrhotic Patients Infected with Carbapenem Resistant Enterobacteriaceae | ||||
Egyptian Journal of Medical Microbiology | ||||
Volume 32, Issue 1, January 2023, Page 1-11 PDF (549.43 K) | ||||
Document Type: New and original researches in the field of Microbiology. | ||||
DOI: 10.21608/ejmm.2023.277762 | ||||
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Authors | ||||
fatma o khalil 1; Sally Saad Mandour Saad Mandour2; Isis Samy Bedira2; Sally Elkhadry3; Amr Ragab Ibrahim4; Neamat Abdelmageed5; Eman El -shemy5; Hala Ahmed El-refai6 | ||||
1Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom, Egypt | ||||
2Clinical Pathology Department, National Liver Institute Menoufia University | ||||
3Epidemiology and Preventive Medicine, National Liver Institute Menoufia University | ||||
4Hepatology and Gastroenterology Department, National Liver Institute Menoufia University | ||||
55Department of Hepatogastroentrology & Infectious Diseases, Faculty of Medicine for girls, Al-Azhar University | ||||
6Microbiology nd Immunology, National Liver Institute, Menoufia University, Shebin El-kom | ||||
Abstract | ||||
Background: Early detection of bacterial infection in patients with cirrhosis remains a hope for improved prognosis to decrease the morbidity and mortality rate. Objective: To study the diagnostic importance of Neutrophil gelatinase-associated lipocalin-2 and macrophage antigen -1 for early detection of infection in cirrhotic patients and their relationship with carbapenem resistant bacteria. Methodology: 150 patients were divided into group I: cirrhotic patients complicated with infection subdivided into three subgroups; A: fifty-five patients with spontaneous bacterial peritonitis (SBP), Subgroup B: fifteen patients with culture-negative neutrocytic ascites (CNNA) and subgroup C: thirty patients with urinary tract infection. Group II: fifty cirrhotic patients without infections. NGAL-2 estimation was done by ELISA, neutrophil MAC-1 by flowcytometry and Carbapenem resistance genes; OXA-48 and KPC-3 were detected by RFLP-PCR analysis Results: Significant increase of ascitic fluid neutrophils and WBCs count in SBP subgroup. Mean fluorescence intensity of Mac-1 and NGAL-2 concentrations were significantly higher in cirrhotic infected than uninfected patients. The OXA-48 and KPC-3 Carbapenem resistant gram-negative bacteria were prevalent among cirrhotic infected patients. NGAL-2 can detect early infection in cirrhotic patients followed by Mac-1. Conclusion: NGAL-2 and Mac-1 could be used for early detection of bacterial infection in cirrhotic patients with high specificity and sensitivity. | ||||
Keywords | ||||
Mac-1; NGAI-2; SBP; PCR; flow cytometer; Cirrhosis | ||||
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