Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales
Aboulela, A., Jabbar, M., Hammouda, A., Ashour, M. (2023). Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales. EKB Journal Management System, 32(1), 37-46. doi: 10.21608/ejmm.2023.277771
Aliaa Gamaleldin Aboulela; Mawj Fahad Jabbar; Abdelfattah Hammouda; Medhat Saber Ashour. "Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales". EKB Journal Management System, 32, 1, 2023, 37-46. doi: 10.21608/ejmm.2023.277771
Aboulela, A., Jabbar, M., Hammouda, A., Ashour, M. (2023). 'Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales', EKB Journal Management System, 32(1), pp. 37-46. doi: 10.21608/ejmm.2023.277771
Aboulela, A., Jabbar, M., Hammouda, A., Ashour, M. Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales. EKB Journal Management System, 2023; 32(1): 37-46. doi: 10.21608/ejmm.2023.277771

Assessment of Phenotypic Testing by mCIM with eCIM for Determination of the type of Carbapenemase Produced by Carbapenem-resistant Enterobacterales

Egyptian Journal of Medical Microbiology
Volume 32, Issue 1, January 2023, Page 37-46  XML PDF (440.82 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.277771
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Authors
Aliaa Gamaleldin Aboulela email orcid 1; Mawj Fahad Jabbarorcid 2; Abdelfattah Hammoudaorcid 3; Medhat Saber Ashourorcid 3
1Medical Research Institute, Alexandria University
2High Institute of public health, Alexandria University
3Microbiology department, High Institute of Public Health, Alexandria University, Alexandria, Egypt
Abstract
Background: Carbapenemase-producing Enterobacterales are increasingly spreading in healthcare facilities. Identifying the type of carbapenemase can help epidemiologic surveillance and proper selection of antimicrobials. Objective: This study assessed the sensitivity and specificity of carbapenem inactivation method (mCIM with eCIM) for identification of carbapenemase-production. Methodology: The study involved 150 isolates of Enterobacterales. Carbapenem-resistant isolates by Kirby Bauer method were further tested for carbapenemase production phenotypically by mCIM with eCIM, and genotypically by multiplex PCR using specific primers for blaKPC, blaOXA-48, blaNDM-1, blaVIM, and blaIMP. Results: Resistance to carbapenem was observed in 53/150 isolates. Phenotypically, 28/53 isolates produced metallo-β-lactamase, 16/53 produced serine carbapenemase, 5/53 isolates gave inconclusive results, and 4/53 were negative by mCIM with eCIM test. Genotypically, 30 isolates carried blaNDM-1, and 41 isolates carried blaOXA-48. Both genes co-existed in 18 Metallo-β-lactamase producers. The 9 isolates with negative or inconclusive results carried carbapenemase-encoding genes. For mCIM with eCIM test the sensitivity and specificity of detecting Metallo-β-lactamase production were higher (87% and 91%) than for serine carbapenemase detection (34% and 83%), respectively. Conclusion: It was concluded that the mCIM with eCIM test does not always lead to true conclusions about the existence and the type of carbapenemase produced by Enterobacterales.
Keywords
Carbapenem-resistant Enterobacterales; mCIM with eCIM; metallo-beta-lactamase detection; serine-carbapenemase detection; blaOXA-48 gene; blaNDM-1 gene
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