Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis
Fathi, F., Kamal, A., Attallah, M., Salah El Deen, N. (2023). Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis. EKB Journal Management System, 32(1), 47-53. doi: 10.21608/ejmm.2023.277776
Fatma EL Zahraa Yousef Fathi; Amany Mostafa Kamal; Makram Fahmy Attallah; Noha Nagi Salah El Deen. "Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis". EKB Journal Management System, 32, 1, 2023, 47-53. doi: 10.21608/ejmm.2023.277776
Fathi, F., Kamal, A., Attallah, M., Salah El Deen, N. (2023). 'Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis', EKB Journal Management System, 32(1), pp. 47-53. doi: 10.21608/ejmm.2023.277776
Fathi, F., Kamal, A., Attallah, M., Salah El Deen, N. Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis. EKB Journal Management System, 2023; 32(1): 47-53. doi: 10.21608/ejmm.2023.277776

Detection of Mutations Associated with Resistance to Second-line Drugs in Isolates of Mycobacterium tuberculosis

Egyptian Journal of Medical Microbiology
Volume 32, Issue 1, January 2023, Page 47-53  XML PDF (496.04 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.277776
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Authors
Fatma EL Zahraa Yousef Fathi1; Amany Mostafa Kamal2; Makram Fahmy Attallah1; Noha Nagi Salah El Deen email 3
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt
2Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
3Medical Microbiology and Immunology, Faculty of medicine, Ain shams University, Cairo, Egypt
Abstract
Background: Mycobacterium tuberculosis (M. tuberculosis) develops resistance toward second-line anti-tuberculous drugs, mostly through mutations of the chromosome. The mechanism of resistance is complicated and accompanies several genes as rrs and eis promoter mutations. Objective: To assess the validity of the Real-time PCR (Rt-PCR) in recognizing the mutations of the resistance to second-line injectable agents (capreomycin, amikacin and kanamycin) in multidrug resistant tuberculosis (MDR-TB) clinical isolates in comparison with the gold standard proportion method (PM) using Lowenstein–Jensen (LJ) media. Methodology: This study was conducted on 48 MDR-TB clinical isolates (from sputum and bronchioalveolar lavage samples) obtained from the Egyptian National Central Laboratory of the Ministry of Health, Egypt. The isolates obtained were resistant to a minimum one of the second-line anti-tuberculous drugs (ofloxacin, capreomycin, amikacin, and kanamycin) identified by PM using LJ media. Isolates were tested by Rt-PCR to track mutations in rrs and the eis promoter. Results: Thirty-eight (38) isolates were positive for rrs, and 32 isolates for the promoter of eis using Rt-PCR. Comparing the results to the gold standard PM, an agreement of 100%, and 69% were found to rrs, and the eis promoter, respectively. Conclusion: Using the Real-time PCR for recognizing mutations related to second-line anti-tuberculous drugs highly agrees with the PM. This could help in MDR-TB early detection and screening for extensively drug-resistant TB (XDR-TB) strains.
Keywords
M. tuberculosis; MDR-TB; Rt-PCR; rrs; promoter of eis
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