Characterization of β-Lactamase Genes among Multidrug- and Extensively Drug-Resistant Acinetobacter and Enterobacteriaceae Species Causing Infections in Critically Ill Patient | ||||
Egyptian Journal of Medical Microbiology | ||||
Volume 32, Issue 1, January 2023, Page 113-123 PDF (681.96 K) | ||||
Document Type: New and original researches in the field of Microbiology. | ||||
DOI: 10.21608/ejmm.2023.277787 | ||||
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Authors | ||||
Reham Ali Khalifa 1; Hani Aljahdali2; Huda Alghamdi3; Bothayna Ismail4; Husam Joharjy5; Khalid Eibani6 | ||||
1Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University | ||||
2Medical Department, King Abdulaziz Hospital, Jeddah, Saudia Arabia | ||||
3Laboratory Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia | ||||
4Clinical Pathology Department, Faculty of Medicine, Al Azhar University, Egypt. | ||||
5Infection Control Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia | ||||
6Surgery Department, King Abdulaziz Hospital, Jeddah, Saudi Arabia | ||||
Abstract | ||||
Background: Multidrug resistant infection is the leading cause of ICU morbidity and mortality. Gram-negative bacilli are the most prevalent pathogens with Acinetobacter being the most resistant. Objectives: Phenotypic and genotypic characterization of multidrug- and extensively drug-resistant Acinetobacter and Enterobacteriaceae species causing infections in critically ill patients with detection of targeted β-lactamase genes and its impact on patients' morbidity and mortality. Methodology: Antibiotic susceptibility testing was done. Genes for class A and class B β-lactamases were detected by multiplex polymerase chain reaction (PCR) and 5 carbapenemases genes were detected by real time PCR using GeneXpert Carba-R system. Results: 16.6 % of A. baumannii were MDR, 50% were XDR, 50% of K. pneumoniae were XDR and 100% of P. stuartii were XDR. Detected genes included blaSHV, blaCTX-M, blaGIM-1, blaSIM-1, blaOXA-48 and blaNDM-1. Conclusion: MDR/XDR infections had a significant impact on patients’ mortality. Molecular epidemiology is crucial to guide infection control committees about preventive measures that can contain MDR/XDR infections and prevent their detrimental effect on patients’ outcome. | ||||
Keywords | ||||
Antimicrobial; Resistance; Gram-negative; Carbapenemase; Carbapenems | ||||
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