Polymorphisms in the exon-3 of leptin gene using tetra-primer ARMS-PCR in Egyptian buffalo | ||||
| Al-Azhar Journal of Agricultural Research | ||||
| Article 16, Volume 47, Issue 2, December 2022, Page 182-185 PDF (446.33 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajar.2022.277854 | ||||
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| Author | ||||
F. A. Sharara 
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| Animal Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| The Egyptian buffalo is one of the Egyptian genetic resources which is irreplaceable and must be preserved, because of its great adaptability to local harsh environmental conditions and resistance to infectious diseases. A total number of 74 blood samples of female Egyptian buffalo were used to assess the allelic and genotypic frequency of G>A SNP at codon 159 of leptin gene. The tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-primer ARMS-PCR), a single nucleotide polymorphism (SNP) technique allows for the simultaneous detection of alleles using a single PCR reaction and gel electrophoresis. Results revealed three genotypes GG, AG, and AA with frequencies of 0.49, 0.39, and 0.12, respectively. Allele frequencies of G and A were found to be 0.69 and 0.31, respectively. For the first time, allelic and genotypic frequency of G>A SNP at codon 159 in Egyptian buffalo was detected with the highest allelic frequency of allele A (0.31). Further studies are needed to provide more information about the association between this SNP and production and reproduction traits. | ||||
| Keywords | ||||
| Egyptian buffalo; Tetra-primer ARMS-PCR; Leptin hormone | ||||
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