Protective effect of Nigella sativa seed extracts against oxytetracycline induced liver and kidney injuries in albino rats
EL-Akkad, N., Sarwat, M., Ali, N., Sawy, M. (2022). Protective effect of Nigella sativa seed extracts against oxytetracycline induced liver and kidney injuries in albino rats. EKB Journal Management System, 47(2), 186-197. doi: 10.21608/ajar.2022.277856
N. M. EL-Akkad; M. I. Sarwat; N. E. Ali; M. S. Sawy. "Protective effect of Nigella sativa seed extracts against oxytetracycline induced liver and kidney injuries in albino rats". EKB Journal Management System, 47, 2, 2022, 186-197. doi: 10.21608/ajar.2022.277856
EL-Akkad, N., Sarwat, M., Ali, N., Sawy, M. (2022). 'Protective effect of Nigella sativa seed extracts against oxytetracycline induced liver and kidney injuries in albino rats', EKB Journal Management System, 47(2), pp. 186-197. doi: 10.21608/ajar.2022.277856
EL-Akkad, N., Sarwat, M., Ali, N., Sawy, M. Protective effect of Nigella sativa seed extracts against oxytetracycline induced liver and kidney injuries in albino rats. EKB Journal Management System, 2022; 47(2): 186-197. doi: 10.21608/ajar.2022.277856

Protective effect of Nigella sativa seed extracts against oxytetracycline induced liver and kidney injuries in albino rats

Al-Azhar Journal of Agricultural Research
Article 17, Volume 47, Issue 2, December 2022, Page 186-197  XML PDF (1.64 MB)
Document Type: Original Article
DOI: 10.21608/ajar.2022.277856
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Authors
N. M. EL-Akkad email 1; M. I. Sarwat2; N. E. Ali2; M. S. Sawy2
1Agriculture Biochemistry Department, Faculty of Agriculture Ain Shams University,Cairo, Egypt.
2Agriculture Biochemistry Department, Faculty of Agriculture Ain Shams University,Cairo, Egypt
Abstract
This research aimed to determine the antioxidant activity and protective effects of Nigella sativa seed extracts against liver and kidney injuries induced by oxytetracycline (OTC) in albino rats. Oil extract (NSO) was prepared by cold pressing that contains fixed and essential oil. Essential oil (EO) was also prepared by hydro-distillation; its contents were identified as twenty-six terpenoids. Ethanolic extract (NSET) was also prepared and its contents of phenols and flavonoids were quantified. Antioxidant activity of both NSO and NSET were measured using DPPH and ABTS. The protective effect of each of NSO and NSET against oxidative stress was determined in Albino rats. Results showed that the liver and kidney weight gain at the end of experiment were decreased in the both extracts while increased in the OTC-treated group. Enzymatic antioxidants, SOD, CAT, and G-px, showed a substantial inhibition in OTC-treated group compared with extract-treated groups. Liver function and kidney function parameters as well as malondialdehyde (MDA) level increased significantly in OTC-treated group while they decreased in extract-treated groups. The preventive properties of the extracts were validated in histological investigation of liver and kidney.
Keywords
Kidney function; Liver function; Oxidative stress
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