Prostatic estimation of time passed since death; oxidative stress markers, histological and immunohistochemical examination (an experimental study) | ||||
| Ain Shams Journal of Forensic Medicine and Clinical Toxicology | ||||
| Volume 40, Issue 1, January 2023, Page 22-33 PDF (1.42 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajfm.2023.278007 | ||||
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| Authors | ||||
| Dalia Ali; Mennatoulah Ahmed | ||||
| Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine-Menia University, Menia Egypt. | ||||
| Abstract | ||||
| Introduction: One of the most essential duties in forensic medicine is determining the postmortem interval. Objectives: The study's purpose is to estimate time passed since death in relation to oxidative stress markers, histopathological changes, and prostatic specific antigen in the prostate. Methods: Fifty mature male albino rats were classified into 5 main groups. Rats were used for determining postmortem changes and oxidative stress markers at five different postmortem intervals (0, 1, 5, 10, 15 days) with prostatic specific antigen (PSA) immunohistochemistry in the prostate. Results: Prostatic gland oxidant parameters (MDA, NO) revealed a significant increase while its antioxidant parameters (GSH, SOD) showed a significant decrease with increased postmortem time. The prostate gland showed normal histology at 0 hours postmortem (PM). While, at 1 day, it revealed minimal autolysis (<5%). Starting from 5 days, moderate autolysis (10–50%) was seen. Ten to fifteen days, moderate to severe autolysis (≥50%) appeared in most of the samples. Staining for PSA showed high expression (++) at 0 hours PM. Moreover, a significant decrease in the amount of PSA’s stained areas that revealed low expression (+) was detected at 1 and 5 days PM. Negative (−) or no immunoreactivity for PSA has been detected at 10 and 15 days PM. Conclusion: Prostatespecific antigen proved to be a useful parameter consistent with the histopathological changes for the estimation of postmortem interval from the prostate. It is approved that the derived oxidative stress markers equations can be helpful in detecting the postmortem interval in forensic science Received in original form: 21 September 2022 Accepted in a final form: 11December 2022 | ||||
| Keywords | ||||
| Postmortem interval; Oxidative stress markers; Prostatic specific antigen; Immunohistochemistry | ||||
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