TRIALS ON THERAPEUTIC EFFECT OF NIGELLA SA TI VA OIL EXTRACT ON CRYPTOSPORIDIUM PARVUM IN EXPERIMENTALLY INFECTED MICE | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 29, Volume 81, Issue 2, July 2003, Page 805-816 PDF (3.2 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2003.278043 | ||||
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| Author | ||||
| MAGDA A. H. EL-REFAII | ||||
| Animal Health Research Institute, Agricultural Research Centre, Ministry of Agriculture, Giza, Egypt | ||||
| Abstract | ||||
| This study was carried out to determine the therapeutic effect of Nigelle saliva oil extract on mice experimentally infected with Cryptosporidium parvum. For this purpose. 25 susceptible swiss mice (2-3 weeks old) were divided into 3 groups. Group I included infected 11 treated mice, group II included 10 infected non-treated ones, and group III composed of 4 non-infected non-treated mice. One week before experimental infection, all mice were immunosupressed by a mixture of 2 mg/t. dexamethasone sodium phosphate and SOO mg/L tetracycline hydrochloride In daily fresh drinking water. Experimental infection of mice was carried out with cryptospoddium pawum oocysts isolated from naturally infected calves. One week later, mice of group I were orally treated with Nigelle oil at a dose of 1 m1/100g body weight for each mouse. Faecal pellets from rectum of each mouse in all groups were examined every other day post-treatment by the Modified Ziehl-Neelsen technique. 0e-cysts intensities were scored in 100 randomly selected fields. The results revealed that mice of group III were refractory, while, mice of group I showed a reduction in oocysts count when compared to those of group II. On day 35th, all mice were sacrificed and parts of their ilea were fixed in 10% formalin solution for histopathotogical examination, as well as, smears from ilea were taken and stained with the Modified Ziehl-Neelsen technique. Villi of ileum of mice in group I showed normal appearance without any changes, while, those of mice of group II showed apparent villus atrophy: shortening and blunting with desquamation of most of villi. Villi of group III were normal. Data were statistically analysed and discussed. | ||||
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