DIAGNOSIS OF HUMAN CYSTIC ECHINOCOCCOSIS BY DETECTING ANTIGEN B IN SERUM AND URINE USING NANOMAGNETIC BEADS-ENZYME LINKED IMMUNOSORBENT ASSAY | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 52, Issue 3, December 2022, Page 491-500 PDF (880.52 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2022.278371 | ||||
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| Authors | ||||
| AMIRA E. KHAYYAL1; MANAL S. E. MAHMOUD1; REHAN B. IBRAHIM1; IBRAHIM R. BAYOUMI2; ABEER F. BADAWY2 | ||||
| 1Department of Parasitology, Faculty of Medicine, Ain-Shams University, Cairo 11566 | ||||
| 2Department of Parasitology, Theodore Bilharz Research Institute, Imbaba 30, Giza, Egypt | ||||
| Abstract | ||||
| Cystic echinococcosis (CE) is usually asymptomatic, and commonly presents by pressure symptoms according to its location. Diagnosis of CE is currently based on imaging techniques, which may not be available in some areas and antibody detection in serum, with its known several drawbacks. Conversely, antigen detection has proven its efficacy in serodiagnosis of CE. The study evaluated the efficacy of Nanomagnetic beads (NMB)-sandwich ELISA for diagnosis of CE by detection of Echinococcus (E.) granulosus Antigen B (AgB) in serum and urine samples as compared with sandwich ELISA. A total of 42 individuals were classified into 3 groups; CE infection group, other parasitic infections group and control group. E. granulosus AgB was prepared from human hydatid cysts to produce anti-E. granulosus AgB-immunoglobulin (Ig) Gpoly- clonal Abs in rabbit, after purification, to detect AgB level in serum and urine samples by sandwich ELISA and NMB-sandwich ELISA. Using sandwich ELISA, AgB was detected in sera of CE patients with sensitivity and specificity of 88.9% & 91.7%, respectively. In urine samples, sandwich ELISA detected AgB in CE patients and the sensitivity and specificity were 83.3% and 87.5%, respectively. NMB-sandwich ELISA detected AgB in serum samples of CE patients with a sensitivity and specificity of 94.4% and 95.8%, respectively, whereas in urine samples it was detected in CE cases and the sensitivity and specificity were 88.9% and 95.8%, respectively, without significant statistical difference (p>0.05) between serum and urine samples for detection of AgB when examined by either sandwich ELISA or NMB-sandwich ELISA. The best accuracy was obtained with NMB-sandwich ELISA detecting AgB in serum samples (95.2%), with a slightly lower accuracy of 92.9% when using urine samples. NMBsandwich ELISA is efficient for detection of E. granulosus AgB in serum samples. Moreover, urine samples might be a good alternative to serum samples for the detection of AgB. | ||||
| Keywords | ||||
| Antigen B; cystic echinococcosis; E. granulosus; hydatid cyst; Nanomagnetic beads-sandwich ELISA; sandwich ELISA; serum; Urine | ||||
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