AMELIORATING EFFECT OF NITAZOXANIDE LOADED CHITOSAN NANOPARTICLES ON INTESTINAL DYSPLASTIC CHANGES IN IMMUNOSUPPRESSED CRYPTOSPORIDIOSIS MURINE MODEL | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 52, Issue 3, December 2022, Page 509-518 PDF (1.08 MB) | ||||
| DOI: 10.21608/jesp.2022.278374 | ||||
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| Authors | ||||
| HOWAYDA S.F. MOAWAD; MOHAMED H.A. HEGAB; MAHA S.R. BADAWEY; SHAIMAA E. ASHOUSH; AMIRA A.S. ALI; SHEREEN M. IBRAHIM | ||||
| Department of Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt | ||||
| Abstract | ||||
| Cryptosporidiosis is a major cause of human diarrhea worldwide in immunocompromised individuals causing severe, chronic and possibly life threatening diarrhea. Nitazoxanide (NTZ) has been approved for treatment of diarrhea in immunocompetent children and adults, but not effective in immunosuppressed individuals. The present study evaluated the effect of NTZ-loaded chitosan nanoparticles on intestinal dysplastic changes of experimental Cryptosporidium infection in immunosuppressed murine model using parasitological, histopathological and immunohistochemical studies. Fifty immunosuppressed male mice, divided into 5 groups (10 each) as following: G1: non infected control, G2: non-treated infected control, G3: infected then treated by nitazoxanide, G4: infected then treated by nitazoxanide loaded on chitosan nanoparticles, G5: infected then treated by chitosan nanoparticles (CS NPs). NTZ loaded on chitosan NPs treated mice showed the highest significant reduction in oocysts shedding, a remarkable improvement in histopathological changes and the least expression of cyclin D1 marker denoting the best protection presented to intestinal dysplastic changes caused by Cryptosporidium infection of the intestine followed by treatment with NTZ then by treatment with CS NPs alone that showed some improvement in histopathological and immunohistochemical changes. | ||||
| Keywords | ||||
| Chitosan nanoparticles; Nitazoxanide; Cryptosporidium; immunosuppression and Dysplasia | ||||
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