EVALUATION OF MULTIPLEX REAL-TIME PCR IN DETECTION OF ENTERIC PATHOGENIC PROTOZOA AMONG DIARRHEIC CHILDREN | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 52, Issue 3, December 2022, Page 519-526 PDF (4.28 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2022.278375 | ||||
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| Authors | ||||
| NAGLAA F. ABD EL-LATIF1; MONA H. EL-SAYAD1; MOHAMMAD ABDELRAHMAN2; TALAL SAEED3; OMAR M. HAMEED1; HEBA SAID IBRAHIM1 | ||||
| 1Department of Parasitology, Medical Research Institute, Alexandria University, Egypt | ||||
| 2Department of Clinical Pathology, Medical Military Academy, Cairo,11291, Egypt | ||||
| 3Department of Laboratory Medicine, Faculty of Medicine and Health Science, University of Amran, Yemen | ||||
| Abstract | ||||
| Gastrointestinal protozoa are one of the global health problems, particularly in the Eastern Mediterranean Countries. Urgent parasitological diagnosis is indicated to start proper treatment. The present study evaluated multiplex real-time (RT) PCR and microscopic examination for detection of some pathogenic protozoa mainly Cryptosporidium parvum, Entamoeba histolytica/ dispar and Giardia lamblia among diarrheic school aged children. The morning stool samples of 100 diarrheic children were examined by stained microscopy smears and multiplex RT-PCR for gastrointestinal intestinal protozoa. The results showed that among the positive children 67/100 by microscopy the detected protozoa were G. lamblia followed by Blastocystis hominis, E. histolytica/dispar, C. parvum and lastly Cyclospora spp. Using multiplex RT-PCR G. lamblia and C. parvum were detected in 40 and 2 stool samples respectively. Twelve samples of E. histolytica/dispar detected by microscopy were negative by multiplex real time PCR probably due to the non-pathogenic E. dispar | ||||
| Keywords | ||||
| Children; Protozoa diarrhea; Microscopy; Multiplex real-time PCR | ||||
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