EVALUATION OF OLIBANUM EXTRACT ACTIVITY AGAINST TRICHINELLA SPIRALIS ADULTS AND LARVAE: IN VITRO STUDY | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 52, Issue 3, December 2022, Page 557-564 PDF (1.23 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2022.278386 | ||||
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| Authors | ||||
| SAWSAN S. SHENDI1; AMIRA M. MATAR2 | ||||
| 1Department of Clinical and Molecular Parasitology, Faculty of Medicine, Menoufia University, Menoufia, 32511, Egypt | ||||
| 2National Liver Institute and Department of Medical Parasitology, Faculty of Medicine, Menoufia University, Menoufia, 32511, Egypt | ||||
| Abstract | ||||
| Trichinellosis is a globally re-emerging parasitic disease. The available current treatments are more or less not effective especially for larvae with many drawbacks. Consequently, appropriate natural treatment is needed. The present study evaluated the in-vitro anthelminthic effects of Olibanum (OL) extract against Trichinella spiralis adults and larvae. Adults and muscle larvae were incubated with OL extract at concentrations of 10, 25, 50, 100, & 150μg/ml on culture medium. The histopathological changes and mortality both stages were assessed by using scanning electron microscopy (SEM). The OL extract showed significant anthelminthic activity against adult and larval stages. The maximum and the earliest inhibitory effect were with 150μg/ml (100% mortality at 36hr incubation) for both stages. However, a concentration of 100μg/ml showed prolonged incubation time (48hr). Difference between both concentrations was significant (P≤ 0.5). Besides, T. spiralis adults and larvae incubated with OL extract at concentrations of 100 &150μg/ml exhibited various histopathological changes in the form of cracks, blebs and areas of degenerative changes with loss of normal annulations. | ||||
| Keywords | ||||
| T. spiralis; Olibanum; In-vitro; Anthelminthic; Scanning electron microscopy | ||||
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