Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I
ZIckri, M., Metwally, H., Abdelwahed, O., Gouda, M., Hassan, R., Zaki, S., Aboulkhair, A. (2024). Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I. EKB Journal Management System, 47(1), 223-237. doi: 10.21608/ejh.2023.177550.1824
Maha Baligh ZIckri; Hala Gabr Metwally; Omaima Mohamed Abdelwahed; Mai Abdelaziz Gouda; Rokia Mohamad Hassan; Shaimaa Ahmed Zaki; Alshaymaa Gamal Aboulkhair. "Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I". EKB Journal Management System, 47, 1, 2024, 223-237. doi: 10.21608/ejh.2023.177550.1824
ZIckri, M., Metwally, H., Abdelwahed, O., Gouda, M., Hassan, R., Zaki, S., Aboulkhair, A. (2024). 'Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I', EKB Journal Management System, 47(1), pp. 223-237. doi: 10.21608/ejh.2023.177550.1824
ZIckri, M., Metwally, H., Abdelwahed, O., Gouda, M., Hassan, R., Zaki, S., Aboulkhair, A. Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I. EKB Journal Management System, 2024; 47(1): 223-237. doi: 10.21608/ejh.2023.177550.1824

Possible Therapeutic Effect of Gene Modified Versus Non-Modified Stem Cells on Hippocampus and Cerebral Cortex in Alzheimer’s Female Albino Rat Model Complicating Experimentally Induced Diabetes Type I

Egyptian Journal of Histology
Article 15, Volume 47, Issue 1, March 2024, Page 223-237  XML PDF (3.47 MB)
Document Type: Original Article
DOI: 10.21608/ejh.2023.177550.1824
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Authors
Maha Baligh ZIckri1; Hala Gabr Metwally2; Omaima Mohamed Abdelwahed3; Mai Abdelaziz Gouda4; Rokia Mohamad Hassanorcid 5; Shaimaa Ahmed Zaki email orcid 6; Alshaymaa Gamal Aboulkhair7
1Medical histolgy departement,Faculty of medicine ,Cairo university.
2Clinical Pathology Faculty of Medicine Cairo University
3Medical Physiology, Faculty of Medicine, Cairo University
4Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University
5Histology and cell biology Faculty of Medicine Cairo University
6Department of Medical Histology and Cell Biology, Cairo University, Egypt
7Histology and cell biology, Faculty of Medicine, Cairo University
Abstract
Introduction and Objectives: The relation linking type1 diabetes (T1DM) to Alzheimer’s disease (AD) was recorded. Recently, widespread attention for gene modification of stem cells (SCs) was established. The present work aimed at investigating possible ameliorating impact of gene modified SCs on cerebral cortex and hippocampus in Alzheimer’s disease (AD) complicating T1DM.
Methods and Results: 40 female rats were classified into: In Vitro Study Group: 4 animals used to perform SCs culture and Sarco-Endoplasmic Reticulum Ca2+ATPase (SERCA) 2b preparation. Control Group (A): 6 animals. Diabetic Group (B): 10 animals received IP injection 50 mg/kg streptozotocin (STZ). Non-transfected SCs Group (C): 10 rats, received 1×106 rat SCs intravenous. SERCA2b transfected SCs Group (D): 10 rats received 1x106 transfected SCs. 52 days after proving incidence of diabetes, neurological, serum glucose, morphological, morphometric, gene quantitation, western blot and biochemical studies were performed. Neurological study expressed a decrease in group B and in group C. Group B clarified degenerated neurons in external pyramidal (EP) layer and dentate gyrus (DG). Group D revealed more obvious regression of degenerative changes than group C. An increase in area of deformed nerve cells and Congo red +ve neurons, mean area% of immunoexpression (IE) were found in groups B and C. Mean Ca ion concentration, catalase, SERCA2b gene and protein values expressed a significant (sig) decrease in groups B and C. Mean MDA and beta amyloid protein proved a sig increase in groups B and C.
Conclusions: Stem cells transfected with sarcoplasmic reticulum calcium ATPase2b gene proved pronounced therapeutic impact suggesting its potency and regenerative capacity in remarkable amelioration of Alzheimer’s disease complicating diabetes I.
Keywords
AMSCs; caspase3; catalase; GFAP; MDA; qPCR; SERCA; T1DM; Wb
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