The effect of the polymorphism in IL-28B gene on the treatment response of a combined therapy by sofosbuvir and daclatasvir with and without ribavirin in HCV Egyptian patients | ||||
Fayoum University Medical Journal | ||||
Volume 11, Issue 1, January 2023, Page 11-18 PDF (611.62 K) | ||||
Document Type: Full Length research Papers | ||||
DOI: 10.21608/fumj.2023.278457 | ||||
View on SCiNiTO | ||||
Authors | ||||
Eman E. Mahmoud1; Hoiyda A. Abdel-Rasoul2; Eriny H. I. Abdallah 3; Essam A. Hassan4 | ||||
1Clinical and chemical pathology department, Fayoum university. | ||||
2Department of Clinical and Chemical Pathology, Faculty of Medicine, Fayoum University, Fayoum, Egypt. | ||||
3Department of clinical and chemical pathology, Faculty of medicine, Fayoum University, Fayoum, Egypt. | ||||
4Tropical medicine departemt, Fayoum university. | ||||
Abstract | ||||
Introduction: HCV infection is a universal health condition. Polymorphisms at the interleukin 28B (IL28B) gene severely affect treatment results to direct-acting antiviral drugs (DAA), including Sofosbuvir and Daclatasvir with or without Ribavirin. The relationship between the virus and the host immune system is critical for revealing the prognosis and the response to medications. Aim of our study: It aimed to reveal if there is an association between the SNP of the IL-28B (rs12979860) gene and hepatitis C virus treatment response to DAA in an Egyptian population. Subjects and methods: This case-control descriptive analytical thesis contains 200 subjects, 100 HCV patients who received Sofosbtuvir, Dacatasvir ± Ribavirin (50responders&50 non-responders) compared to 100 age and sex-matched healthy control subjects. Diseased people were recruited from the Tropical clinic in Fayoum University Hospital from May 2019 to February 2020. All samples were subjected to genomic DNA analysis of SNP of the IL-28B gene (rs12979860) using Real-Time PCR. Results: The SNP IL-28B gene (rs12979860) was not statistically significantly different between HCVdiseased people compared to healthy subjects (P > 0.05). The c allele was also higher among drugresponsive patients, while the T allele was higher among drug-resistant patients (P <0.01), which is a highly statistically significant difference. Conclusion: The study showed a significant relation between IL-28Bgene (rs12979860) polymorphism and hepatitis C virus treatment response to DAA. CC genotype is a significant indicator of SVR in HCV genotype 4 Egyptian patients, and this genotype may influence the effectiveness of treatment. | ||||
Keywords | ||||
IL-28B gene; polymorphism; sustained virological response | ||||
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