GC-MS Analysis of Bioactive Compounds of Gardenia thunbergia Thunb. Leaves and Antibiofilm Potential against Staphylococcus aureus Clinical Isolates
EL-Shial, E., Kabbash, A., El-Aasr, M., Elekhnawy, E., El-Sherbeni, S. (2023). GC-MS Analysis of Bioactive Compounds of Gardenia thunbergia Thunb. Leaves and Antibiofilm Potential against Staphylococcus aureus Clinical Isolates. EKB Journal Management System, 4(1), 11-18. doi: 10.21608/jampr.2023.170762.1048
Eman M. EL-Shial; Amal Kabbash; Mona El-Aasr; Engy Elekhnawy; Suzy A. El-Sherbeni. "GC-MS Analysis of Bioactive Compounds of Gardenia thunbergia Thunb. Leaves and Antibiofilm Potential against Staphylococcus aureus Clinical Isolates". EKB Journal Management System, 4, 1, 2023, 11-18. doi: 10.21608/jampr.2023.170762.1048
EL-Shial, E., Kabbash, A., El-Aasr, M., Elekhnawy, E., El-Sherbeni, S. (2023). 'GC-MS Analysis of Bioactive Compounds of Gardenia thunbergia Thunb. Leaves and Antibiofilm Potential against Staphylococcus aureus Clinical Isolates', EKB Journal Management System, 4(1), pp. 11-18. doi: 10.21608/jampr.2023.170762.1048
EL-Shial, E., Kabbash, A., El-Aasr, M., Elekhnawy, E., El-Sherbeni, S. GC-MS Analysis of Bioactive Compounds of Gardenia thunbergia Thunb. Leaves and Antibiofilm Potential against Staphylococcus aureus Clinical Isolates. EKB Journal Management System, 2023; 4(1): 11-18. doi: 10.21608/jampr.2023.170762.1048

GC-MS Analysis of Bioactive Compounds of Gardenia thunbergia Thunb. Leaves and Antibiofilm Potential against Staphylococcus aureus Clinical Isolates

Journal of Advanced Medical and Pharmaceutical Research
Volume 4, Issue 1, March 2023, Page 11-18  XML PDF (392.9 K)
Document Type: Original Article
DOI: 10.21608/jampr.2023.170762.1048
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Authors
Eman M. EL-Shial email 1; Amal Kabbashorcid 2; Mona El-Aasrorcid 2; Engy Elekhnawy3; Suzy A. El-Sherbeniorcid 2
1Department of pharmacognosy, Faculty of Pharmacy, Horus University, New Damietta, Egypt
2Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta, Egypt
3Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Tanta University, El Giesh Street, Tanta,Egypt
Abstract
The goal of this study was to utilize gas chromatography-mass spectrometry to characterize the bioactive phytochemicals found in the petroleum ether fraction of Gardenia thunbergia Thunb. leaves methanol extract. The GC-MS analysis yielded several peaks, indicating the existence of 23 compounds tentatively with various medicinal properties. Palmitic acid, butyl palmitate, acetyl tributyl citrate, methyl palmitate, cholesta-4,6-dien-3-ol, and ursolic acid methyl ester were found to be the most prevalent compounds. In this study, the antibacterial effect of total methanol extract and petroleum ether fraction against Staphylococcus aureus was evaluated and compared with the positive control (vancomycin) and the negative control (dimethyl sulfoxide). Twenty-two S. aureus clinical isolates were obtained for the experiment. The values of MICs were detected, and the effect of the tested extracts on the biofilm formation ability of S. aureus isolates was evaluated. The total methanol extract and the petroleum ether fraction have notable antibacterial activity against the tested S. aureus clinical isolates.
Keywords
Bioactive compounds; Gardenia thunbergia; GC-MS; Petroleum ether fraction; Staphylococcus aureus
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