Total Phenolic Content and Antimicrobial Activity of Mentha rotundifolia L. from Western Algeria

Nesrine, Bouyakoub,; Khedoudja, Kanoun,; Sara, Bouchouicha,; Zeyneb, Nourine,; Zohra, EL-Kadi, Fatima; Noria, Harir,; Bouziane, Abbouni,; Aicha, Megharbi,

doi:10.21608/eajbsc.2022.278611

	Total Phenolic Content and Antimicrobial Activity of Mentha rotundifolia L. from Western Algeria
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 14, Issue 2, December 2022, Page 427-438 PDF (771.86 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2022.278611
View on SCiNiTO
Authors
Bouyakoub, Nesrine¹; Kanoun, Khedoudja¹; Bouchouicha, Sara¹; Nourine, Zeyneb¹; EL-Kadi, Fatima Zohra²; Harir, Noria¹; Abbouni, Bouziane¹; Megharbi, Aicha²
¹Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.
²Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.
Abstract
The objective of the present study is to the total phenolic compound determination, as well as the in vitro evaluation of methanolic and ethanolic extracts antimicrobial activity of the medicinal plant Mentha rotundifolia L. against pathogenic strains. The Follin-Cioacalteu method was used to determine the total polyphenols, while the flavonoids were estimated according to the colorimetric method with aluminum chloride. In addition, the determination of condensed tannins is performed by the vanillin method. The antimicrobial activity is tested on five pathogenic bacterial strains, two of which are Gram-negative (Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853), three Gram-positive (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Micrococcus leutus ATCC 4698), and one yeast (Candida albicans ATCC 26790), by the diffusion on solid medium and microdilution methods. The ethanolic extract of round leaf mint from the Bechar region is very rich in polyphenols and flavonoids (401.75 ± 0.32 mg GAE/g, 549.75 ± 1.23 mg QE/g) compared to the methanolic and hydroalcoholic extracts of this plant from the Sidi-Bel-Abbes region. The results of the obtained well method showed that all bacterial strains exhibited sensitivity to the ethanolic extract of Mentha rotundifolia L. from the Bechar region, with inhibition zones up to 21,67 ± 0,57 mm and minimum inhibitory concentrations (MIC) varying between 06.25 mg/ml and 100 mg/ml, The effect of the other extracts on the tested strains was less significant, the methanolic extract was the least effective. These results demonstrate that the Mentha rotundifolia L. ethanolic extract has a great potential of secondary metabolites with antimicrobial activity.
Keywords
Mentha rotundifolia L; ethanolic extract; methanolic extract; phenolic compounds; pathogenic strains; antimicrobial activity


Statistics Article View: 157 PDF Download: 176