Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina
Singab, A., Ibrahim, N., Elsayed, A., El-Senousy, W., Aly, H., Abd Elsamiae, A., Matloub, A. (2018). Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina. EKB Journal Management System, 2(2), 121-137. doi: 10.21608/aps.2018.18740
Abdel Nasser Singab; Nabaweya Ibrahim; Abo El-khair Elsayed; Waled El-Senousy; Hanan Aly; Asmaa Abd Elsamiae; Azza Abdelmageed Matloub. "Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina". EKB Journal Management System, 2, 2, 2018, 121-137. doi: 10.21608/aps.2018.18740
Singab, A., Ibrahim, N., Elsayed, A., El-Senousy, W., Aly, H., Abd Elsamiae, A., Matloub, A. (2018). 'Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina', EKB Journal Management System, 2(2), pp. 121-137. doi: 10.21608/aps.2018.18740
Singab, A., Ibrahim, N., Elsayed, A., El-Senousy, W., Aly, H., Abd Elsamiae, A., Matloub, A. Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina. EKB Journal Management System, 2018; 2(2): 121-137. doi: 10.21608/aps.2018.18740

Antiviral, cytotoxic, antioxidant and anti-cholinesterase activities of polysaccharides isolated from microalgae Spirulina platensis, Scenedesmus obliquus and Dunaliella salina

Archives of Pharmaceutical Sciences Ain Shams University
Article 6, Volume 2, Issue 2, June 2018, Page 121-137  XML PDF (1.15 MB)
Document Type: Original Article
DOI: 10.21608/aps.2018.18740
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Authors
Abdel Nasser Singab1; Nabaweya Ibrahim2; Abo El-khair Elsayed3; Waled El-Senousy4; Hanan Aly5; Asmaa Abd Elsamiae6; Azza Abdelmageed Matloub email 2
11Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Abassia, Egypt
2Pharmacognosy Dept., National Research Centre, Cairo, Egypt
3Algal Biotechnology Unit, National Research Centre, 33 Bohouth Street, Dokki, Cairo, Egypt
4Water Pollution Research Department (Virology Lab), National Research Centre, 33 Bohouth Street, Dokki, Cairo, Egypt.
5Therapeutic Chemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Cairo, Egypt
6Pharmacognosy Department, National Research Centre, 33 Bohouth Street, Dokki, Cairo, Egypt
Abstract
Quantitative estimation of vegetative and stress forms of Spirulina platensis and Scenedesmus obliquus as well as vegetative form of Dunaliella salina revealed that S. obliquus constituted highest polysaccharide content than other tested microalgae. The isolated polysaccharides characterized as heterogeneous polysaccharides bounded protein by FT-IR, GLC and Elemental Microanalysis. These polysaccharides constituted of 47-66% of sugar and 14.88-41.06% of protein contents whereas galactose, mannose, glucose and rhamnose were represented as predominant sugar in isolated polysaccharides.
The isolated polysaccharides were evaluated in vitro as antiviral, cytotoxic, antioxidant and anti-cholinesterase properties. The non-toxic dose of isolated polysaccharides on Huh 7.5, MA104, BGM and Vero cell lines were determined. The S. platensis (CEM and HEM) polysaccharides have promising antiviral which reduced replication up to 50–87.6% of HCV genotype 4a replicon, coxsackievirus B4, rotavirus and herpes simplex type 1 virus at non toxic doses 1.8 and 1.5 mg/ml, respectively.
Furthermore, the isolated polysaccharides were assessed for in-vitro cytotoxic activity against MCF-7, HepG2 and HCT116 cell lines. The cytotoxic activity revealed that D. salina HEM polysaccharide show potent cytotoxic activity against HCT 116 cell line with IC50 64.2 μg/ml. Additionally, the isolated polysaccharides showed DPPH• scavenging activity in dose dependent relationship and D. salina HEM and S. obliquus CEM showed the significantly highest activity (308.16 and 308.69%, respectively) at 100 μg/ml. Furthermore, S. obliquus CEM and HEM polysaccharides exhibited the significant highest cholinestearse % inhibition activity.
Microalgal polysaccharides have great therapeutically potential in drug development used as antiviral, antitumour, antioxidant and anticholinestrae agents in near future.
Keywords
microalgae; Polysaccharides; antiviral activity; Cytotoxic activity; antioxidant and anti-cholinesterase
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