Therapeutic Effects of Exosomes Released from Adipose- Derived Mesenchymal Stem Cells on a Male Rat Model of Ulcerative Colitis; Histological study | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 14 January 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.182707.1837 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Samia Hamdy Radwan  1; Mohammed Hafez Ahmed Moustafa2; Nevan Said Mahmoud Abd ElKareem3; Abeer Ibraheem Omar 4
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| 1histology ,faculty of medicine ,cairo university | ||||
| 2histology department, faculty of medicine , cairo university | ||||
| 3Histology, Faculty of Medicine, Cairo University | ||||
| 4Histology department, Faculty of Medicine, Cairo University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Ulcerative colitis (UC) is a global health problem with high incidence and morbidity rates. It is considered as one of the most common diseases of large intestine in human even in pediatric population. The goal of this work is to evaluate the potential therapeutic effects of exosomes derived from adipose MSCs on a male rat model of acetic acid induced colitis. Methods: 39 rats divided into 3 groups: Group (I) Control (n= 13 rats). Group (II) colitis (n=13) received intra-colonic dose of 2 ml of 4% acetic acid once: 3 of them were sacrificed at day 2, the remaining were divided into 2 subgroups: (IIa) (5 rats), and (IIb) (5 rats): sacrificed at the 9th and 16th day of the experiment respectively. Group (III) exosomes treated (n= 13): as group II, then at day 2 each rat received single intravenous dose of ADMSCs-E. 24 hours later (day 3) 3 rats were sacrificed. The remaining were divided into 2 subgroups: (IIIa) (5 rats), and (IIIb) (5 rats): sacrificed with the subgroups IIa and IIb, respectively. Colonic sections were stained with Hematoxylin & Eosin, Alcian blue as well as immunohistochemical staining for NF-κB as well as biochemical homogenate for TNF-α. Morphometry and statistical analysis were done. Results: Colitis group showed deterioration of rats’ general condition, ulceration of colonic mucosa, goblet cells depletion, inflammatory cell infiltration with increased TNF-α and NF-κB immunostaining. Restoration of colonic mucosa and goblet cells with decreased TNF-α, NF-κB immunostaining were detected in exosomes treated group. Conclusion: Exosomes had the ability to attenuate acetic acid induced colitis to an extent comparable to normalization. | ||||
| Keywords | ||||
| Ulcerative Colitis; Acetic Acid; Exosomes; TNF-α; NF-κB | ||||
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