Effect of Green Tea and Black Seed on Methotrexate Induced Cytotoxicity of the Oral Mucosa, Tongue and the Submandibular Salivary Gland of Albino Rats
Fathi, R., Abd Allah, D., Helmy, I. (2023). Effect of Green Tea and Black Seed on Methotrexate Induced Cytotoxicity of the Oral Mucosa, Tongue and the Submandibular Salivary Gland of Albino Rats. EKB Journal Management System, 69(1), 413-426. doi: 10.21608/edj.2022.175561.2352
Rehab Fathi; Dalia Abd Allah; Iman Helmy. "Effect of Green Tea and Black Seed on Methotrexate Induced Cytotoxicity of the Oral Mucosa, Tongue and the Submandibular Salivary Gland of Albino Rats". EKB Journal Management System, 69, 1, 2023, 413-426. doi: 10.21608/edj.2022.175561.2352
Fathi, R., Abd Allah, D., Helmy, I. (2023). 'Effect of Green Tea and Black Seed on Methotrexate Induced Cytotoxicity of the Oral Mucosa, Tongue and the Submandibular Salivary Gland of Albino Rats', EKB Journal Management System, 69(1), pp. 413-426. doi: 10.21608/edj.2022.175561.2352
Fathi, R., Abd Allah, D., Helmy, I. Effect of Green Tea and Black Seed on Methotrexate Induced Cytotoxicity of the Oral Mucosa, Tongue and the Submandibular Salivary Gland of Albino Rats. EKB Journal Management System, 2023; 69(1): 413-426. doi: 10.21608/edj.2022.175561.2352

Effect of Green Tea and Black Seed on Methotrexate Induced Cytotoxicity of the Oral Mucosa, Tongue and the Submandibular Salivary Gland of Albino Rats

Egyptian Dental Journal
Article 23, Volume 69, Issue 1 - Serial Number 3, January 2023, Page 413-426  XML PDF (1.42 MB)
Document Type: Original Article
DOI: 10.21608/edj.2022.175561.2352
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Authors
Rehab Fathi email orcid 1; Dalia Abd Allahorcid 2; Iman Helmyorcid 3
1Lecturer of Oral Pathology, Oral Pathology Department, Faculty of Dentistry, Ain Shams University
2Lecturer of Oral Pathology, Ahram Canadian University
3Professor and Head of Oral Pathology Department, Faculty of Dentistry, Ain Shams University
Abstract
Abstract:

Background: Methotrexate (MTX) is a drug which has a wide application in the treatment of malignant diseases and autoimmune diseases. The main side effect of MTX is oral mucositis as well as salivary gland damage. Objective: to evaluate and compare the individual effect of green tea and black seeds oil extract, on MTX induced oral mucositis and submandibular salivary gland cytotoxicity in albino rats.
Material and Methods: A total of fifty six male Albino rats were used, divided into four experimental groups, Group 1:control group, Group 2: MTX treated group, Group 3: MTX + Green Tea extract treated group and Group 4: MTX + Black seed oil treated group. Rats were sacrificed separately after five and ten days. Specimens were processed for H/E staining and immunohistochemically prepared for COX-2 expression. The data was analyzed and expressed statistically.
Results: Group 1 showed normal structure of the salivary gland, the oral and tongue mucosa. Group 2 showed severe loss in the normal histologic structure of the tissues with marked inflammatory cell infiltration. Group 3 showed almost normal epithelium with reduction in the inflammatory cell infiltrate. Group 4 also showed reduction in signs of inflammation with areas of fibrosis. Immunohistochemical results revealed a weak positive reaction of COX 2 in groups 1, 3 and 4 while a strong positive reaction was noticed in group 2.
Conclusion: The green tea caused marked improvement in the tissues, with obvious anti-inflammatory effect after five days of treatment. Further improvement was noticed after ten days.
Keywords
MTX; salivary glands; green tea; black seed; COX-2
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