Role of Soluble CD163 and Monocyte Surface Markers in Hepatitis C Virus Infected Patients
El Sayed, L., Ghoniem, H., Awda, S., Nayer, S., El Tayeb, R., Osman, E. (2022). Role of Soluble CD163 and Monocyte Surface Markers in Hepatitis C Virus Infected Patients. EKB Journal Management System, 43(1), 8-17. doi: 10.21608/jmalexu.2022.281702
Laila H El Sayed; Hossam Ghoniem; Said Awda; Salwa Nayer; Reham El Tayeb; Eman Osman. "Role of Soluble CD163 and Monocyte Surface Markers in Hepatitis C Virus Infected Patients". EKB Journal Management System, 43, 1, 2022, 8-17. doi: 10.21608/jmalexu.2022.281702
El Sayed, L., Ghoniem, H., Awda, S., Nayer, S., El Tayeb, R., Osman, E. (2022). 'Role of Soluble CD163 and Monocyte Surface Markers in Hepatitis C Virus Infected Patients', EKB Journal Management System, 43(1), pp. 8-17. doi: 10.21608/jmalexu.2022.281702
El Sayed, L., Ghoniem, H., Awda, S., Nayer, S., El Tayeb, R., Osman, E. Role of Soluble CD163 and Monocyte Surface Markers in Hepatitis C Virus Infected Patients. EKB Journal Management System, 2022; 43(1): 8-17. doi: 10.21608/jmalexu.2022.281702

Role of Soluble CD163 and Monocyte Surface Markers in Hepatitis C Virus Infected Patients

Journal of the Medical Research Institute
Article 2, Volume 43, Issue 1, June 2022, Page 8-17  XML PDF (623.2 K)
Document Type: Review Article
DOI: 10.21608/jmalexu.2022.281702
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Authors
Laila H El Sayed1; Hossam Ghoniem1; Said Awda2; Salwa Nayer3; Reham El Tayeb4; Eman Osman email 1
1Department of Immunology and Allergy, Medical Research Institute, Alexandria University
2Department of Internal Medicine, Medical Research Institute, Alexandria University
3Department of Oncology, Medical Research Institute, Alexandria University
4Reseaech departnent for tissue engineering,Faculty of dentistry,Alexandria University
Abstract
Background: Chronic stimulation of circulating monocytes and macrophages in hepatitis C virus infection can cause CD163 to be realesed into the circulation from their cell surface. Soluble CD163 (sCD163) can be used as a biomarker of macrophage activation, severity of HCV infection and level of inflammation.
Objective: In this study we determined the levels of soluble CD163 in HCV infected patients with different clinical outcomes and we correlated them to different biochemical indicators for disease progression and monocytic cell surface markers (CD14, CD16).
Methods: Our study was conducted on 60 HCV infected patients:20 patients with chronic HCV infection without cirrhosis; 20 patients with cirrhosis,20 patients with hepatocellular carcinoma and 20 individuals who were healthy controls. Peripheral blood mononuclear cells (PBMCs) were isolated from all subjects and were used in flow cytometric determination of CD14 and CD16 monocytic surface markers . Levels of sCD163 in serum and culture supernatents were determined by enzyme linked immunosorbent assay.
Results: Our results demonstrated a significant increase in double positive monocytic cells in patients groups especially those with cirrhosis compared to the control group. Moreover soluble CD163 levels were significantly increased in HCV patients with different clinical outcomes. The relation between serum CD163 and double positive monocytes was shown to be significantly negative. However, there was a substantial positive connection between these monocytic surface markers and mitogen-induced CD163.
Conclusion: Our findings suggest that HCV infection tends to upregulate CD163 shedding into circulation with a variety of clinical outcomes, allowing sCD163 to be employed as a biomarker for disease severity in HCV infection.
Keywords
soluble CD163; HCV infection; mitogen induced sCD163; CD14+CD16+ monocytes
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