Improvement of kidney injury molecule-1 (KIM-1) and N-acetylglucosamindase (NAG) in the kidney following platelet rich plasma (PRP) administration in the treatment of cyclosporine A-induced nephrotoxicity in adult male albino rats | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 24 January 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2023.183942.1840 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Tarek Ibrahim Abdel Galil1; Soheir Helmy Elsharouny2; Mohamed Hafez Shaaban3; Hadeer Maher Tohamy  4; Sarah Mahmoud Kaooh2
| ||||
| 1Cairo university, faculty of medicine, anatomy department | ||||
| 2Anatomy department, faculty of medicine, Cairo university | ||||
| 3, Professor of Anatomy and Embryoloy, Faculty of Medicine, Cairo University. | ||||
| 4Anatomy, kasr Alainy, Cairo university | ||||
| Abstract | ||||
| Background: Cyclosporin A (CsA) is an immunosuppressive drug used to treat transplant rejection and autoimmune diseases. However, adverse effects limit its use in clinical practice. PRP is an autologous growth factor-rich product thought to be useful as a regenerative product by releasing growth factors that alleviate tissue damage. Material and Methods: Forty adult male albino rats were used in the study, divided into four equal groups; control, sham control, CsA-treated, Cyclosporine A-PRP treated. Histological (H & E and Masson’s trichrome), immunohistochemical (caspase-3 and TGFβ-1), biochemical assessment (serum urea, create, tissue level of Glutathione reductase, SOD and MDA, and tissue expression of KIM-1 and NAG) were performed. Results: In CsA treated group, renal cortex revealed shrunken segmented glomerulus with darkly stained nuclei, widened urinary space, interstitial hemorrhage, and intraluminal casts. Other sections showed hypercellular glomerulus with extremely narrow urinary space, intraglomerular hemorrhage, tubular vacuolations and karyolysis. Also, there was excessive deposition of collagen fibers around and within glomeruli as well as around tubules in group III. Immunohistochemically, Sections from group III showed strong positive reaction of caspase-3 and TGFβ-1. Biochemically there is elevated serum urea, creatinine, and tissue MDA level together with decreased tissue level SOD and Glutathione reductase in group III. Regarding gene expression there was increased genetic expression of KIM-1 and NAG in group III. PRP could ameliorate histopathological nephrotoxic effects induced by CsA. As well as improving biochemical markers and gene expression values. Conclusion: PRP appeared to be a safe product could be used to reverse the adverse effects induced by CsA administration. | ||||
| Keywords | ||||
| cyclosporin A; PRP; kidney; KIM-1; NAG | ||||
|
Statistics Article View: 115 |
||||
