In Vitroand in Silico studies of Neophytadiene; A Diterpene Isolated Fromaeschynomene Elaphroxylon (Guill. &Perr.) Taub. as Apoptotic Inducer
Selmy, A., Hegazy, M., El-Hela, A., Saleh, A., El-Hamouly, M. (2023). In Vitroand in Silico studies of Neophytadiene; A Diterpene Isolated Fromaeschynomene Elaphroxylon (Guill. &Perr.) Taub. as Apoptotic Inducer. EKB Journal Management System, 66(10), 149-161. doi: 10.21608/ejchem.2023.178261.7296
Ahmed Hany Selmy; Mostafa Mahmoud Hegazy; Atef Ahmed El-Hela; Abdulrahman M. Saleh; Mohamed M. El-Hamouly. "In Vitroand in Silico studies of Neophytadiene; A Diterpene Isolated Fromaeschynomene Elaphroxylon (Guill. &Perr.) Taub. as Apoptotic Inducer". EKB Journal Management System, 66, 10, 2023, 149-161. doi: 10.21608/ejchem.2023.178261.7296
Selmy, A., Hegazy, M., El-Hela, A., Saleh, A., El-Hamouly, M. (2023). 'In Vitroand in Silico studies of Neophytadiene; A Diterpene Isolated Fromaeschynomene Elaphroxylon (Guill. &Perr.) Taub. as Apoptotic Inducer', EKB Journal Management System, 66(10), pp. 149-161. doi: 10.21608/ejchem.2023.178261.7296
Selmy, A., Hegazy, M., El-Hela, A., Saleh, A., El-Hamouly, M. In Vitroand in Silico studies of Neophytadiene; A Diterpene Isolated Fromaeschynomene Elaphroxylon (Guill. &Perr.) Taub. as Apoptotic Inducer. EKB Journal Management System, 2023; 66(10): 149-161. doi: 10.21608/ejchem.2023.178261.7296

In Vitroand in Silico studies of Neophytadiene; A Diterpene Isolated Fromaeschynomene Elaphroxylon (Guill. &Perr.) Taub. as Apoptotic Inducer

Egyptian Journal of Chemistry
Volume 66, Issue 10, October 2023, Page 149-161  XML PDF (2.17 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.178261.7296
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Authors
Ahmed Hany Selmy email orcid 1; Mostafa Mahmoud Hegazyorcid 2; Atef Ahmed El-Hela1; Abdulrahman M. Salehorcid 3; Mohamed M. El-Hamouly1
1Department of Pharmacognosy and medicinal plants, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt.
2Department of Pharmacognosy and medicinal plants, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, 11884-Egypt
3Department of Pharmaceutical Medicinal Chemistry & Drug Design, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt.
Abstract
Aeschynomene elaphroxylon (Guill. & Perr.) Taub. is a large aquatic shrub native to Madagascar and tropical Africa and cultivated in Egypt. Petroleum ether extract showed remarkable cytotoxic activities against human lung carcinoma (A-549), and prostate carcinoma cells (PC-3) with IC50 values 12.3±0.8 and 23.8±1.2 μg/ml, respectively. So, pet. ether extract was furtherly fractionated affording seven fractions, tested for cytotoxic activity against A-549, and PC-3. The sub-fraction (PET-5) from pet. ether fraction showed potent cytotoxic activities more than cisplatin against A-549, and PC-3 with IC50 values 6.94±0.48 and 7.33±0.58 µg/ml, respectively. The major compound of sub-fraction PET-5 was identified by GCMS as Neophytadiene (1) at percentage of 82.23 %. In silico studies showed that neophytadiene blocks three receptors which have main role in cancer viability, invisibility as it blocks Human A2a receptor at adenosine binding site, Human LRH1 and hERG K+ channel leads to inhibition of cancer proliferation and invasiveness which may act as a promising apoptotic inducer. The phytochemical investigation of the plant major fractions resulted in isolation of five compounds. From pet. ether fraction (1) Neophytadiene was identified, from ethyl acetate fraction (2) Kaempferol-7-O-α-L-rhamnoside and (3) Medicarpin-3-O-β-D- glucopyranoside were isolated and from n-butanol fraction (4) kaempferol 3-O-β-D-apiofuranosyl-7-O-alpha-L-rhamnoside, (5) Kaempferol 3,7-di-O-α-L-rhamnoside were isolated.
Keywords
Neophytadiene; Diterpene; Cytotoxicity; Apoptosis; Docking; Fabaceae
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