Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice
Sanad, M., Marzook, F., Challan, S., Essam, H., Farag, A. (2023). Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice. EKB Journal Management System, 56(3), 105-120. doi: 10.21608/ajnsa.2022.163538.1639
Mahmoud H Sanad; Fawzy Abdel Megeed Marzook; Safaa Bekheet Challan; Heba Mahmoud Essam; Ayman B. Farag. "Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice". EKB Journal Management System, 56, 3, 2023, 105-120. doi: 10.21608/ajnsa.2022.163538.1639
Sanad, M., Marzook, F., Challan, S., Essam, H., Farag, A. (2023). 'Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice', EKB Journal Management System, 56(3), pp. 105-120. doi: 10.21608/ajnsa.2022.163538.1639
Sanad, M., Marzook, F., Challan, S., Essam, H., Farag, A. Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice. EKB Journal Management System, 2023; 56(3): 105-120. doi: 10.21608/ajnsa.2022.163538.1639

Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice

Arab Journal of Nuclear Sciences and Applications
Volume 56, Issue 3, April 2023, Page 105-120  XML PDF (853.76 K)
Document Type: Original Article
DOI: 10.21608/ajnsa.2022.163538.1639
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Authors
Mahmoud H Sanad1; Fawzy Abdel Megeed Marzook2; Safaa Bekheet Challan email 3; Heba Mahmoud Essam4; Ayman B. Farag5
1Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority,P.O. Box 13759, Cairo, Egypt
2Labelled compound compartment- Hot Lab. Center, Atomic Energy Authority
3Cyclotron Project, Nuclear Research Center, Atomic Energy Authority, Inshas , Egypt
4Cyclotron Project, Nuclear Research Center, Atomic Energy Authority, Inshas , Egypt. P.O Box 13759
53Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, Giza, 12578 Egypt
Abstract
Ulcerative colitis (UC) is a chronic, regressive nature disease. The use of conventional diagnostic procedures such as magnetic resonance imaging, ultrasonography, and X-rays during the dormant and early stages of the disease does not aid in diagnosing the disease. As a result, a novel design, such as labelled compounds, was used to image ulcerative colitis disease. In this study, olsalazine was labeled with [125/131I] and the labelling parameters were adjusted to obtain a high radiochemical yield (98.5%). In addition, the olsalazine radiotracer gave 96.0% purity in rate serum for up to twelve hours before it started to degrade at twenty-four hours, it was stable also, in saline for up to twenty-four hours. Molecular docking was used to assess a complex's affinity for its biological target, and the PPAR receptor. We also performed the biological assessment in mice models of both standard and ulcerative colitis. Results demonstrated that [125/131I] iodoolsalazine had a high uptake of 79.5% (ID/g) at 120 minutes post-injection and still high to 77.00% at 24 hours. So, the labelled compound, [131I] iodoolsalazine, could be considered a new potential selective radiotracer for preclinical diagnostic research of ulcerative colitis.
Keywords
Keywords: Olsalazine; Iodine-125/131; (PPARγ) Peroxisome Proliferator-Activated Receptors Gamma; Biological Assessment; Imaging Ulcerative Colitis
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