Biofilm Formation of Staphylococcus aureus Isolated from Infected Wound
Mohamed, M., Mohammed, A., Goda, A., Mohamed, S. (2019). Biofilm Formation of Staphylococcus aureus Isolated from Infected Wound. EKB Journal Management System, 28(2), 97-103. doi: 10.21608/ejmm.2019.282702
Mona F. Mohamed; Alaa El Din H. Mohammed; Asmaa M. Goda; Samah R. Mohamed. "Biofilm Formation of Staphylococcus aureus Isolated from Infected Wound". EKB Journal Management System, 28, 2, 2019, 97-103. doi: 10.21608/ejmm.2019.282702
Mohamed, M., Mohammed, A., Goda, A., Mohamed, S. (2019). 'Biofilm Formation of Staphylococcus aureus Isolated from Infected Wound', EKB Journal Management System, 28(2), pp. 97-103. doi: 10.21608/ejmm.2019.282702
Mohamed, M., Mohammed, A., Goda, A., Mohamed, S. Biofilm Formation of Staphylococcus aureus Isolated from Infected Wound. EKB Journal Management System, 2019; 28(2): 97-103. doi: 10.21608/ejmm.2019.282702

Biofilm Formation of Staphylococcus aureus Isolated from Infected Wound

Egyptian Journal of Medical Microbiology
Volume 28, Issue 2, April 2019, Page 97-103  XML PDF (340.86 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.282702
View on SCiNiTO View on SCiNiTO
Authors
Mona F. Mohamed email 1; Alaa El Din H. Mohammed2; Asmaa M. Godaorcid 1; Samah R. Mohamed1
1Microbiology and Immunology Department, Faculty of Medicine, Sohag University
2General Surgery Department, Faculty of Medicine, Sohag University
Abstract
Background: Staphylococcus aureus has the ability to form biofilms, and causes significant mortality and morbidity in the patients with wounds. Objective: Our aim was to study the in vitro biofilm-forming ability of isolated S. aureus. Methodology: One hundred clinical isolates of S. aureus were isolated from 350 pus samples using standard microbiological techniques. Biofilm formation ability of these isolates was detected phenotypically by tissue culture plate (TCP) method and congo red agar (CRA) and genotypically by detection of ica ABCD genes by PCR. Results: The clinical isolates of S. aureus recovered from infected wounds exhibited a high degree of biofilm formation. Biofilm formation was observed in (76%), (74%) and (70%) of S. aureus isolates via TCP method CRA and genotypically, respectively. Conclusion: This study illustrated that PCR method can be adopted as the most suitable and reproducible method for detection of biofilm. CRA is qualitative, simple, inexpensive and easily reproducible and convenient as a screening method. TCP is semiquantitative method and remains a precious tool for in vitro screening of different biomaterial for the adhesive properties. Regular surveillance of biofilm formation by S. aureus leads to the early treatment of the wound infection.
Keywords
Biofilm; Staphylococcus aureus; wounds; ica ABCD genes
Statistics
Article View: 52
PDF Download: 65