Role of Melatonin, Glutamine and L-arginine in Prevention of Non-alcoholic Fatty Liver Disease in Rats
Obydah, W., Elwakeel, G., Abd El-Hamed, A., Gad, G., El Menabawy, F. (2019). Role of Melatonin, Glutamine and L-arginine in Prevention of Non-alcoholic Fatty Liver Disease in Rats. EKB Journal Management System, 39(1), 35-51. doi: 10.21608/besps.2018.4413.1005
Walaa O. Obydah; Gehan A. Elwakeel; Aya E. Abd El-Hamed; Gad Elmawla A. Gad; Fayza R. El Menabawy. "Role of Melatonin, Glutamine and L-arginine in Prevention of Non-alcoholic Fatty Liver Disease in Rats". EKB Journal Management System, 39, 1, 2019, 35-51. doi: 10.21608/besps.2018.4413.1005
Obydah, W., Elwakeel, G., Abd El-Hamed, A., Gad, G., El Menabawy, F. (2019). 'Role of Melatonin, Glutamine and L-arginine in Prevention of Non-alcoholic Fatty Liver Disease in Rats', EKB Journal Management System, 39(1), pp. 35-51. doi: 10.21608/besps.2018.4413.1005
Obydah, W., Elwakeel, G., Abd El-Hamed, A., Gad, G., El Menabawy, F. Role of Melatonin, Glutamine and L-arginine in Prevention of Non-alcoholic Fatty Liver Disease in Rats. EKB Journal Management System, 2019; 39(1): 35-51. doi: 10.21608/besps.2018.4413.1005

Role of Melatonin, Glutamine and L-arginine in Prevention of Non-alcoholic Fatty Liver Disease in Rats

Bulletin of Egyptian Society for Physiological Sciences
Article 3, Volume 39, Issue 1, June 2019, Page 35-51  XML PDF (1.09 MB)
Document Type: Original Article
DOI: 10.21608/besps.2018.4413.1005
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Authors
Walaa O. Obydah1; Gehan A. Elwakeel email 2; Aya E. Abd El-Hamed3; Gad Elmawla A. Gad4; Fayza R. El Menabawy1
1Medical Physiology Department, Faculty of Medicine, Mansoura university, Mansoura, Egypt
2Medical Physiology Department, Mansoura Faculty of Medicine, Mansoura, Egypt
3Medical Experimental Research Center (MERC), Mansoura University,Mansoura, Egypt
4Medical Physiology Department, Faculty of Medicine, Mansoura university, Mansoura, Egypt.
Abstract
Over the next years, non alcoholic fatty liver disease will represent the main cause of chronic liver disease with the reduction of hepatitis C burden. Recent researches are directed towards prevention. Prevention of NAFLD can be achieved by attenuation of oxidative stress. The aim of this work is to study the possible role of melatonin, glutamine and L-arginine in prevention of non-alcoholic fatty liver disease in rats induced by high fat, and high carbohydrate diet.
The study included control, NAFLD, melatonin, glutamine and arginine groups. For all groups we have measured the serum concentration of glucose, lipid profile, liver enzymes, the concentration of glutathione (GSH) and malonyl aldehyde (MDA) in liver tissues. Then we performed histopathological study of liver tissue . there was significant increase in blood glucose level, triglycerides, Cholesterol and LDL in NAFLD, significant increase in liver enzymes (AST, ALT) and MDA, and significant decrease in GSH in NAFLD group as compared with control group.
The use of melatonin, glutamine and L-arginine improved all the parameters as compared with NAFLD group. By histopathological study, marked improvement with slight fatty infiltration and near normal hepatocytes in melatonin group, moderate improvement with mild steatosis in glutamine group while mild improvement with L-arginine group.
From this work we can conclude that: Early intervention with melatonin, glutamine or L-arginine has a protective effect in NAFLD.

Key words: NAFLD, melatonin, L-arginine, glutamine, oxidative stress –
glucose – lipid profile.
Keywords
NAFLD; Melatonin; L-arginine; Glutamine; Oxidative Stress
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