Ameliorative potential of biguanides on experimentally-induced lung fibrosis
borg, H., Abd Elmaaboud, M., Balaha, M., Abdelmonem, F., Abdel-Rahman, M., Hedeya, S. (2019). Ameliorative potential of biguanides on experimentally-induced lung fibrosis. EKB Journal Management System, 39(1), 102-117. doi: 10.21608/besps.2019.7985.1017
hany borg; Maaly Abd Elmaaboud; Mohamed Balaha; Fleur Abdelmonem; Mohamed Abdel-Rahman; Sabiha Hedeya. "Ameliorative potential of biguanides on experimentally-induced lung fibrosis". EKB Journal Management System, 39, 1, 2019, 102-117. doi: 10.21608/besps.2019.7985.1017
borg, H., Abd Elmaaboud, M., Balaha, M., Abdelmonem, F., Abdel-Rahman, M., Hedeya, S. (2019). 'Ameliorative potential of biguanides on experimentally-induced lung fibrosis', EKB Journal Management System, 39(1), pp. 102-117. doi: 10.21608/besps.2019.7985.1017
borg, H., Abd Elmaaboud, M., Balaha, M., Abdelmonem, F., Abdel-Rahman, M., Hedeya, S. Ameliorative potential of biguanides on experimentally-induced lung fibrosis. EKB Journal Management System, 2019; 39(1): 102-117. doi: 10.21608/besps.2019.7985.1017

Ameliorative potential of biguanides on experimentally-induced lung fibrosis

Bulletin of Egyptian Society for Physiological Sciences
Article 8, Volume 39, Issue 1, June 2019, Page 102-117  XML PDF (1.56 MB)
Document Type: Original Article
DOI: 10.21608/besps.2019.7985.1017
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Authors
hany borg email 1; Maaly Abd Elmaaboud2; Mohamed Balaha2; Fleur Abdelmonem2; Mohamed Abdel-Rahman2; Sabiha Hedeya2
1faculty of medicine, kafrelsheikh university
2Pharmacology department, faculty of Medicine, Tanta University, Egypt
Abstract
Lung fibrosis is a disease that carries poor prognosis and high mortality rate. The mechanisms of fibrosis may include disordered wound healing, infiltration with inflammatory cells and fibroblasts and release of reactive oxygen species and growth factors. The aim of this study was to assess the effect of metformin (Biguanide) on lung fibrosis induced by bleomycin and to clarify the molecular mechanisms of this effect. Sixty male Wistar rats were divided into 6 equal groups as follows: control group; bleomycin for 4 weeks group; metformin prophylactic group; bleomycin for 6 weeks group; metformin therapeutic group and metformin alone group. The weight of rats was recorded. Bronchoalveolar lavage (BAL) was analyzed for total and differential leukocyte count, tumor necrosis factor alpha (TNF-α) and transforming growth factor beta 1 (TGF-β1). Lung tissue hydroxyproline, malondialdehyde and superoxide dismutase were measured. Also, parts of the lungs were subjected to histopathological and immunohistochemical examination for nuclear factor kappa B (NF-κB). Metformin used prophylactically improved the histopathological picture and NF-κB immunostaining and decreased the oxidative stress, TGF-β1, TNF-α and BAL cellularity. When used therapeutically, metformin decreased oxidative stress and TGF-β1 but didn’t improve TNF-α, the histopathological picture and NF-κB immunostaining. In conclusion, metformin has ameliorative effect on bleomycin-induced lung fibrosis when used prophylactically better than when used therapeutically.
Keywords
biguanides; lung; fibrosis; Bleomycin; Rats
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