THE EFFECT OF HYPOCHLORITE ON SKIN'S MRNA MARKERS IN CONTACT SAMPLES | ||||
| The Egyptian Journal of Forensic Sciences and Applied Toxicology | ||||
| Article 4, Volume 18, Issue 1, May 2018, Page 55-61 PDF (1.14 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejfsat.2018.28294 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| Background: the identification of human skin through mRNA-based profiling is useful in forensic investigations. A deliberate attempt to remove biological material, through using a variety of cleaning agents, is a problem faced by forensic scientists. Limited information exists on the effect of Hypochlorite (HOCl); cleaning agent; on human skin mRNA markers. Aim: This study evaluated the sensitivity of specific human genetic skin markers; LCE2D and CCL27 to different HOCl concentrations by using Real-time qPCR. Method: sixty four middle aged healthy volunteers (32 males and 32 females) were included in the study.Results: HOCl had a pronounced negative effect. The expression of the studied markers was decreased to the quarter of its normal value when the touch samples were submerged in concentrated HOCl solution. Conclusion: sample with reduced RNA expressions might be soaked or adulterated before testing and other tests should be used to help in cellular origin determination | ||||
| Keywords | ||||
| forensic investigation; touch samples; mRNA skin markers; LCE2D; CCL27; hypochlorite; Real-time qPCR | ||||
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