Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals
Ghonaim, M., Mostafa, R., Alkady, S. (2019). Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals. EKB Journal Management System, 28(3), 25-32. doi: 10.21608/ejmm.2019.282956
Mabrouk M Ghonaim; Rasha G Mostafa; Shymaa S Alkady. "Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals". EKB Journal Management System, 28, 3, 2019, 25-32. doi: 10.21608/ejmm.2019.282956
Ghonaim, M., Mostafa, R., Alkady, S. (2019). 'Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals', EKB Journal Management System, 28(3), pp. 25-32. doi: 10.21608/ejmm.2019.282956
Ghonaim, M., Mostafa, R., Alkady, S. Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals. EKB Journal Management System, 2019; 28(3): 25-32. doi: 10.21608/ejmm.2019.282956

Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals

Egyptian Journal of Medical Microbiology
Volume 28, Issue 3, July 2019, Page 25-32  XML PDF (584.86 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.282956
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Authors
Mabrouk M Ghonaim; Rasha G Mostafa; Shymaa S Alkady email
Department of Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt
Abstract
Background: E. coli may develop different complex mechanisms of resistance to various classes of antibiotics. Objectives: To assess the prevalence of E. coli in Menoufia University Hospitals, to evaluate its antibiotic resistance profile and to detect the presence of aminoglycoside resistance genes [aac(3)IIa and aac(6)Ib] by real-time polymerase chain reaction (PCR). Methodology: A total of 200 clinical samples were collected from patients in Menoufia University Hospitals. E. coli was identified by standard microbiological methods. E. coli isolates were tested by the modified Kirby-Bauer disk diffusion method, and for extended-spectrum β-lactamases production. Aac(3)IIa and aac(6)Ib genes were detected by real-time PCR. Results: E. coli forms 25% of the isolated bacterial strains. E. coli isolates showed the highest resistance to pipracillin and amoxicillin-clavulanate (96%). On the other hand, carbapenemes had the least resistance rate (30%) followed by amikacin (44%), gentamicin (60%),
tobramycin (60%) and streptomycin (64%). ESβL production was found among 70% of the isolates by the screening and 50% by the confirmatory methods. Real-time PCR showed that 84% of the isolates contain aac(3)IIa, 52% contain aac(6)Ib and 46% contain both genes. Conclusion: E. coli showed resistance to different classes of antibiotics including aminoglycosides with high prevalence of aac(3)IIa and aac(6)Ib genes. Therefore, infection control measures and rationalized use of antibiotics are mandatory.
Keywords
Aminoglycoside resistance; Escherichia coli; Phenotypic; Molecular Characterization
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