Phenotypic and genotypic characterization of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae isolates from Mansoura University Children Hospital
EL-Ageery, S., Abou El-Khier, N., Zeid, M. (2019). Phenotypic and genotypic characterization of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae isolates from Mansoura University Children Hospital. EKB Journal Management System, 28(3), 105-110. doi: 10.21608/ejmm.2019.283061
Safaa M. EL-Ageery; Noha T. Abou El-Khier; Mayada S. Zeid. "Phenotypic and genotypic characterization of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae isolates from Mansoura University Children Hospital". EKB Journal Management System, 28, 3, 2019, 105-110. doi: 10.21608/ejmm.2019.283061
EL-Ageery, S., Abou El-Khier, N., Zeid, M. (2019). 'Phenotypic and genotypic characterization of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae isolates from Mansoura University Children Hospital', EKB Journal Management System, 28(3), pp. 105-110. doi: 10.21608/ejmm.2019.283061
EL-Ageery, S., Abou El-Khier, N., Zeid, M. Phenotypic and genotypic characterization of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae isolates from Mansoura University Children Hospital. EKB Journal Management System, 2019; 28(3): 105-110. doi: 10.21608/ejmm.2019.283061

Phenotypic and genotypic characterization of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae isolates from Mansoura University Children Hospital

Egyptian Journal of Medical Microbiology
Volume 28, Issue 3, July 2019, Page 105-110  XML PDF (408.22 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.283061
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Authors
Safaa M. EL-Ageery1; Noha T. Abou El-Khier email 1; Mayada S. Zeid2
1Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt
2Infectious Diseases and Malnutrition Unit, Children Hospital, Faculty of Medicine, Mansoura University, Egypt
Abstract
Background: The plasmid mediated AmpC β-lactamases (pAmpCs) production is one of the most essential mechanism of drug resistance in Klebsiella pneumoniae (K. pneumoniae). Objective: Phenotypic and genotypic investigation of plasmid mediated AmpC β -lactamase production in K. pneumoniae and its antimicrobial profile among pediatric patients. Methodology: Ninety-two K. pneumoniae isolates were tested for pAmpC β-lactamase production by cefoxitin disk diffusion test. Multiplex polymerase chain reaction (multiplex PCR) was done to detect plasmid mediated AmpC β-lactamases genes in the cefoxitin resistant isolates. Antimicrobial susceptibility testing was done to pAmpC β- lactamase positive isolates by means of Kirby Bauer disc diffusion method. Results: Out of the 92 K. pneumoniae isolates, 37 strains (40.21%) were resistant to cefoxitin. Plasmid mediated AmpC genes were detected in 67.56% (25/37), ten isolates (40%) had CMY gene, 8 isolates (32%) had DHA gene and 7 isolates (28%) had FOX gene. All of the 25(100%) isolates were resistant to co-amoxiclav, ceftazidime, cefotaxime, cefuroxime, ceftriaxone and cefoxitin and 7 isolates (28%) showed resistance against imipenem with variable susceptibilities to the other antibiotics. Conclusion: Monitoring of Plasmid mediated AmpC β-lactamase producing K. pneumoniae is important because of its high prevalence among pediatric patients. Imipenem was the drug of choice for treatment of such infections.
Keywords
K. pneumoniae; AmpC β-lactamase; plasmid; pediatric patients
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