5Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
El-Melegy, M., Noor ELdien, N., Nooh, H., Elseify, G. (2019). 5Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt. EKB Journal Management System, 28(3), 131-140. doi: 10.21608/ejmm.2019.283180
Manal A. El-Melegy; Nermeen M. Noor ELdien; Hanaa Z. Nooh; Ghada H. Elseify. "5Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt". EKB Journal Management System, 28, 3, 2019, 131-140. doi: 10.21608/ejmm.2019.283180
El-Melegy, M., Noor ELdien, N., Nooh, H., Elseify, G. (2019). '5Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt', EKB Journal Management System, 28(3), pp. 131-140. doi: 10.21608/ejmm.2019.283180
El-Melegy, M., Noor ELdien, N., Nooh, H., Elseify, G. 5Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt. EKB Journal Management System, 2019; 28(3): 131-140. doi: 10.21608/ejmm.2019.283180

5Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt

Egyptian Journal of Medical Microbiology
Volume 28, Issue 3, July 2019, Page 131-140  XML PDF (963.99 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.283180
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Authors
Manal A. El-Melegy email 1; Nermeen M. Noor ELdien2; Hanaa Z. Nooh3; Ghada H. Elseify4
1Departments of Parasitology, Faculty of Medicine, Menoufia University, Egypt
2Department of Anatomy, Faculty of Medicine, Menoufia University
3Departments of 2Anatomy, Faculty of Medicine, Menoufia University
4Departments of Histology &Molecular Biology, Faculty of Medicine, Menoufia University
Abstract
Background: Cryptosporidiosis constitutes one of the most common causes of severe diarrhea in immunocompromised and malnourished individuals. It is considered as a life threatening for both of them. Objective: the present work aimed to assess the effect of priming with 102 Cryptosporidium parvum (C.parvum) oocysts on the immune response in the jejunum of experimental malnourished mice trying to restore the host immune defense against the parasite. Methodology: The experiment lasted for 23 days. Fifty (50) female Swiss mice aged 4weeks were randomly and equally divided into five groups: sham control; infected; malnourished; malnourished –infected and primed protected groups. All groups were followed for weight, oocyst shedding and intestinal parasite tissue load. At the end of the experiment, jejunums were collected and prepared for histological, morphometrical, immunohistochemical and cluster of differentiation CD4 & inducible Nitric Oxide Synthase (iNOS) gene expressions. Results: In compare to malnourished –infected group, primed protected group showed evident clinical, histopathological & immunological improvement. Body weight loss, oocyst shedding & parasite tissue burden were reduced in primed protected group. Also, an increase in iNOS and CD4 gene expressions and immune reactions in primed group reflected as an increased villous length and absence of dysplastic changes in the jejunum compared to malnourished –infected group. Conclusion: These outcomes deepen the insight of potential role of C. parvum priming on both innate and adaptive immunity in malnourished infected mice jejunum for further studies.
Keywords
Cryptosporidiosis; Malnourishment; iNOs; CD4; priming. PCR
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