Toll-like Receptor 7 Expression and Gene Polymorphism in Patients with Hepatitis C and Hepatocellular Carcinoma
Ghonaim, M., Abd El Aziz, A., Mostafa, R., Khalil, F., Mohammed, A. (2019). Toll-like Receptor 7 Expression and Gene Polymorphism in Patients with Hepatitis C and Hepatocellular Carcinoma. EKB Journal Management System, 28(4), 149-156. doi: 10.21608/ejmm.2019.283496
Mabrouk M. Ghonaim; Azza M. Abd El Aziz; Rasha G. Mostafa; Fatma O. Khalil; Asmaa E.M. Mohammed. "Toll-like Receptor 7 Expression and Gene Polymorphism in Patients with Hepatitis C and Hepatocellular Carcinoma". EKB Journal Management System, 28, 4, 2019, 149-156. doi: 10.21608/ejmm.2019.283496
Ghonaim, M., Abd El Aziz, A., Mostafa, R., Khalil, F., Mohammed, A. (2019). 'Toll-like Receptor 7 Expression and Gene Polymorphism in Patients with Hepatitis C and Hepatocellular Carcinoma', EKB Journal Management System, 28(4), pp. 149-156. doi: 10.21608/ejmm.2019.283496
Ghonaim, M., Abd El Aziz, A., Mostafa, R., Khalil, F., Mohammed, A. Toll-like Receptor 7 Expression and Gene Polymorphism in Patients with Hepatitis C and Hepatocellular Carcinoma. EKB Journal Management System, 2019; 28(4): 149-156. doi: 10.21608/ejmm.2019.283496

Toll-like Receptor 7 Expression and Gene Polymorphism in Patients with Hepatitis C and Hepatocellular Carcinoma

Egyptian Journal of Medical Microbiology
Volume 28, Issue 4, October 2019, Page 149-156  XML PDF (525.83 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.283496
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Authors
Mabrouk M. Ghonaim1; Azza M. Abd El Aziz2; Rasha G. Mostafa1; Fatma O. Khalilorcid 2; Asmaa E.M. Mohammed email 2
1Microbiology and Immunology Department, Faculty of Medicine- Menoufia University
2Medical Microbiology and Immunology Department, National Liver Institute, Menoufia University
Abstract
Background: Toll-like receptor-7 (TLR7) plays pivotal roles in type I interferon (IFN) secretion and protective antiviral immunity against hepatitis C virus (HCV) infection. Objectives: To assess expression levels of TLR7 in patients with hepatitis C infection and patients with hepatocellular carcinoma (HCC), to investigate possible association between TLR7 (rs179009) polymorphism and infection outcome, and to compare the expressions of TLR7 variants. Methodology: A total of 98 candidates, divided into four groups, were enrolled in this study. TLR7 mRNA expression level was determined for all participants using real-time PCR, and TLR7 single nucleotide polymorphism (SNP) rs179009 was assessed using Taq-Man SNP genotyping assay and real-time PCR. Results: TLR7 expression levels were significantly lower in patients with chronic HCV and HCC compared to patients who naturally cleared their infection and controls (P< 0.05). In females, the AA genotype and the A allele were significantly predominant in HCV clearance group (83.33%), healthy controls (78.6%) and HCC patients (75%), compared to females with chronic hepatitis (21.4%) (P< 0.05), while, the AG and GG genotypes were more commonly found in chronic HCV-infected females. However, no association was found regarding TLR7 polymorphism genotypes distribution in males and HCV susceptibility. High TLR7 expression levels were associated with the AA genotype in HCV-infected females and males. Conclusion: HCV may induce down regulation of TLR7 expression. Polymorphisms in TLR7 gene with different expression levels might affect immune response during HCV infection.
Keywords
TLR7 expression; TLR7 SNP; HCV; HCC
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