Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing Streptomyces Isolated from Egyptian Localities
Soliman, M., Emam, D., Swailam, H., Swelim, M., Rezk, S. (2022). Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing Streptomyces Isolated from Egyptian Localities. EKB Journal Management System, 35(1), 69-81. doi: 10.21608/ejrsa.2023.167331.1140
Mahmoud A.A. Soliman; Doaa A. Emam; Hisham M. Swailam; Mhmoud A. Swelim; Samar A. Rezk. "Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing Streptomyces Isolated from Egyptian Localities". EKB Journal Management System, 35, 1, 2022, 69-81. doi: 10.21608/ejrsa.2023.167331.1140
Soliman, M., Emam, D., Swailam, H., Swelim, M., Rezk, S. (2022). 'Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing Streptomyces Isolated from Egyptian Localities', EKB Journal Management System, 35(1), pp. 69-81. doi: 10.21608/ejrsa.2023.167331.1140
Soliman, M., Emam, D., Swailam, H., Swelim, M., Rezk, S. Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing Streptomyces Isolated from Egyptian Localities. EKB Journal Management System, 2022; 35(1): 69-81. doi: 10.21608/ejrsa.2023.167331.1140

Molecular, Physiological and Biochemical Identification of Polyhydroxybutyrate Producing Streptomyces Isolated from Egyptian Localities

Egyptian Journal of Radiation Sciences and Applications
Volume 35, Issue 1, December 2022, Page 69-81  XML PDF (3.09 MB)
Document Type: Original Article
DOI: 10.21608/ejrsa.2023.167331.1140
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Authors
Mahmoud A.A. Soliman email orcid 1; Doaa A. Emam2; Hisham M. Swailam2; Mhmoud A. Swelim3; Samar A. Rezk3
1Department of Radiation Microbiology, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA), Cairo, Egypt
2Radiation Microbiology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt
3Botany and Microbiology Department, Faculty of Science, Benha University, Egypt
Abstract
THE AMOUNT of chemosynthetic plastic waste increases every year and the exact time for its degradation is unknown. Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. In the present study, soil samples were collected from the rhizosphere region of seven different crops commonly grown in Egyptian soils and used to collect Streptomyces isolates from Benha, Al-Qalioubia Governorate. A total of 50 representative actinomycete isolates (6 from lettuce, 5 from garlic, 9 from onion, 6 from zucchini, 9 from eggplant, 8 from wheat, 7 from alfalfa rhizosphere soil) were collected and refined. Screening was performed by staining colonies of isolates with Sudan Black B. The dye Sudan Black was used as a screening tool for PHB production. Streptomyces isolates were identified by morphological, biochemical and molecular methods. A phylogenetic tree constructed based on the resulting 16S rRNA-encoding gene sequences was examined. A Streptomyces isolate turned out to be Streptomyces incanus BK128. PHB-purified extract of Streptomyces incanus BK128 was characterized using FTIR in comparison to standard PHB. 
Keywords
PHB; Purified product and SEM; Rhizosphere; Streptomyces incanus BK128; 16s rRNA
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