Screening and Production of Fungal L-Asparaginase Enzymes as Anticancer Agents from High-Contrast Soil Environments in Egypt
Kassab, M. (2023). Screening and Production of Fungal L-Asparaginase Enzymes as Anticancer Agents from High-Contrast Soil Environments in Egypt. EKB Journal Management System, 15(1), 21-41. doi: 10.21608/eajbsg.2023.283740
Mohammed M. Kassab. "Screening and Production of Fungal L-Asparaginase Enzymes as Anticancer Agents from High-Contrast Soil Environments in Egypt". EKB Journal Management System, 15, 1, 2023, 21-41. doi: 10.21608/eajbsg.2023.283740
Kassab, M. (2023). 'Screening and Production of Fungal L-Asparaginase Enzymes as Anticancer Agents from High-Contrast Soil Environments in Egypt', EKB Journal Management System, 15(1), pp. 21-41. doi: 10.21608/eajbsg.2023.283740
Kassab, M. Screening and Production of Fungal L-Asparaginase Enzymes as Anticancer Agents from High-Contrast Soil Environments in Egypt. EKB Journal Management System, 2023; 15(1): 21-41. doi: 10.21608/eajbsg.2023.283740

Screening and Production of Fungal L-Asparaginase Enzymes as Anticancer Agents from High-Contrast Soil Environments in Egypt

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Volume 15, Issue 1, June 2023, Page 21-41  XML PDF (1.05 MB)
Document Type: Original Article
DOI: 10.21608/eajbsg.2023.283740
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Author
Mohammed M. Kassab
Instructor of microbiology and immunology, Department of microbiology and immunology, Faculty of Pharmacy, Cairo University, Egypt.
Abstract
Background: Acute lymphoblastic leukemia represents a great crusader of death for children globally. Aspergillus niger is the source of the L-asparaginase enzyme from various soil environments. Similar to the human L-asparaginase enzyme. L-asparaginase enzyme in bacteria, commonly produced by Erwinia chrysanthemum or Escherichia coli, is currently used for acute lymphoblastic leukemia management and to reduce carcinogenic acrylamide creation during food preparation. Study Objectives: Manufacture of fungal L-asparaginase enzyme as an antineoplastic factor. Methodology: In the current study, fungal L-asparaginase was produced on mineral asparagine agar (MAM) selective medium (only fungi that utilize asparagine as a sole source for carbon and nitrogen can grow on it) at PH 6.5, temperature 250C, and incubation for 3 days. Malt agar medium was used for sub-culturing fungal L-asparaginase-producing strains. The biological activity and cytotoxicity activity were obstinate through nessleriazation and MTT[(dimethylthiazol-2-yl) diphenyl tetrazonium] assays respectively. Results: L-asparaginase in fungi showed high potency and bioavailability as an anticancer agent. According to morphological and biochemical tests and DNA blot hybridization, the major fungal isolate producing this enzyme was Aspergillus niger. The molecular mass was 65 KDa as discovered aside from a mass spectrometer. Vmax of 163.8 UML− 1 min− 1 and a Km value of 3.41 × 10− 3 M were displayed via L-asparaginase. Conclusion: This was a promising approach study and fungal L-asparaginase was generated without hypersensitivity reactions and glutaminase-like effects with superior bioactivity to bacteria.
Keywords
Fungal; L-asparaginase; production; anticancer agent
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