Production and Evaluation of the Immunogenicity of an Enterobacter aerogenes Ghost Vaccine in a Mouse Model

Keshta, Akaber Tarek; Zahran Mohamed, Faten; Hassan, Mahmoud Ayman; Sheweita, Salah Ahmed

doi:10.21608/ejchem.2023.188146.7474

	Production and Evaluation of the Immunogenicity of an Enterobacter aerogenes Ghost Vaccine in a Mouse Model
Egyptian Journal of Chemistry
Volume 66, Issue 10, October 2023, Page 539-547 PDF (3.29 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.188146.7474
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Authors
Akaber Tarek Keshta¹; Faten Zahran Mohamed ²; Mahmoud Ayman Hassan ²; Salah Ahmed Sheweita³
¹Chemistry department, Faculty of Science, Zagazig University, Cairo, Egypt
²Chemistry Department, Faculty of Science, Zagazig University, Egypt.
³Department of Clinical Biochemistry, Faculty of Medicine, King Khalid University, Abha, Saudi Arabia
Abstract
Enterobacter aerogenes is a common cause of hospital-acquired infections such as pneumonia and urinary tract infections. Multidrug-resistant strains have emerged as a public health threat. In this study, we used a non-expensive method called the sponge-like reduced protocol (SLRP) to create E. aerogenes bacterial ghosts EAGs (deactivated bacteria) and evaluated their safety and immunogenicity in mice. The mice were given three doses of EAGs or EAGs with an adjuvant and then challenged with live E. aerogenes bacteria. The results showed that the EAG-immunized mice had higher levels of specific antibodies IgG, IgA, and IgM, lower bacterial load in internal organs after challenge, and improved histopathological examination of the liver and spleen compared to a negative control group. The serum from the EAG-immunized mice also had cross-reactivity with other Enterobacteriaceae pathogens using western blotting and enzyme-linked immunosorbent assay (ELISA). These findings suggest that the EAG vaccine may be an effective and safe alternative for the prevention and control of multidrug-resistant infections caused by E. aerogenes. This study showed that E. aerogenes ghosts could potentially be used as a vaccine.
Keywords
Enterobacter aerogenes; Bacterial ghosts; Vaccine; Immunogenic response; Cross-reactivity; Sponge-like reduced protocol (SLRP)


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