Anti-Cancer Effect of Mentha pulegium L. | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 267, Volume 90, Issue 1, January 2023, Page 1781-1788 PDF (944.06 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2023.284325 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Walaa Najm Abood  ; Anas A. Humadi
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| Abstract | ||||
| Background: Many chemotherapy drugs used for treatment cancer, but has many side effects. A lot of studies assessed natural extract as anti-cancer agent and reduced cells proliferation. Objective: The present study was done to investigate the anti-cancer effect of M. pulegium leaves ethanol extract fractions and investigate the active component by GC-MS spectrum analysis. Methods: Cytotoxicity and anti-cancer abilityofM. pulegium extract was investigated by exposing tumor cell line (MDA-MB-231, MCF7 & PC3) and Human hepatic cell line (WRL68) to different doses from M. pulegium extract. We evaluated the ability of MTHF2 in induction apoptosis process in the treated cancer cell by using AO/EB stain for estimation of apoptotic cells. Results: M. pulegium extract was no toxic effect on the WRL68 while decreasing in the viability percent of treated MCF7, MDA-MB-231 and PC3 cell in doses dependent manner, lost their normal shape to be around, detached floating in culture media with reduction in the number then become under growth inhibition. Alteration in the nuclei of the normal cell was clearly showed by Fluorescent microscope image to cells stained with double AO/EB stain, which nuclei of apoptotic cell appeared fluorescent green, condensed chromatin, DNA fragmented with formation of apoptotic bodies. MTHF2 was applied to GC-MS analysis for diagnose the active compounds, identified twenty two active compounds; the most abundant are Ethyl Oleate in the percentage of 62.68% and Octadecanoic acid, ethyl ester in the percentage of 66.06%. Both compounds have a high antioxidant effect. Conclusion: M. pulegium leaves extract fraction 2 has anti-cancer effect by inhibition the cancer cell proliferation throughout enhanced apoptosis process. | ||||
| Keywords | ||||
| Anti-cancer; Apoptosis; Mentha pulegium L; Anti-proliferation; Experimental study; University of Diyala | ||||
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