Quantitatively Disturbed Larval Haemogram of Galleria mellonella L. (Lepidoptera: Pyralidae) by Certain Plant Growth Regulators | ||||
| Egyptian Academic Journal of Biological Sciences. A, Entomology | ||||
| Volume 16, Issue 1, March 2023, Page 61-87 PDF (612.77 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsa.2023.284942 | ||||
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| Authors | ||||
| Ghoneim, K.1; Hamadah, Kh.1; El-Hela, A.2; Abo Elsoud, A. A.1 | ||||
| 1Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Cairo, Egypt. | ||||
| 2Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt. | ||||
| Abstract | ||||
| The greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) is distributed in different parts of the world. Because of the voracious feeding of larvae and their tunnelling behaviour, this pest destructs the honeycomb and other bee‐hive products. The objective of the present study was to evaluate the impacts of the plant growth regulators (PGRs), viz., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyladenine (6-BA), on the important quantitative characters of the larval haemogram, viz., total hemocyte count (THC) and differential hemocyte counts (DHCs). For this purpose, the 3rd instar larvae were force-fed on diet mixed with LC50 of each PGR (0.24, 0.022, 0.16 & 0.085 ppm, respectively). The haematological investigation was conducted in haemolymph of the 5th and 7th (last) instar larvae. The important results could be summarized as follows. Five main types of freely circulating hemocytes in the haemolymph of the 5th instar and 7th (last) instar larvae had been identified as Prohemocytes, Plasmatocytes, Granulocytes, Spherulocytes and Oenocytoids. The THC in haemolymph of control larvae slightly decreased with the instar. The feeding of 3rd instar larvae on diet mixed with IBA, 2,4-D, or 6-BA resulted in increasing THC in haemolymph of 5th instar larvae. Also, IAA, 2,4-D and 6-BA promoted 7th instar larvae to produce considerably increasing THC. In contrast, IAA exhibited a slight reducing effect on 5th instar larvae, since decreasing THC was counted. Also, IBA exerted a suppressing action on larvae to produce normal THC in 7th instar larvae. The tested PGRs exerted diverse actions on DHCs since no certain trend was detected in the fluctuated hemocyte populations. Moreover, some of the PGRs failed to affect the DHCs of certain hemocyte types. However, the auxin PGR, IAA and IBA, may be effective agents in the IPM program against G. mellonella. | ||||
| Keywords | ||||
| Differential hemocyte count; indole-3-acetic acid; indole-3-butyric acid; 2; 4-Dichlorophenoxy acetic acid; 6-benzyladenine; total hemocyte count | ||||
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