Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen
Younan,, G., Shehab El-Din, A., Abdel-Khalek, A., El-Sherbieny, M., Helmy, A. (2021). Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen. EKB Journal Management System, 12(1), 47-54. doi: 10.21608/jappmu.2021.149460
G. E. Younan,; A. M. Shehab El-Din; A. E. Abdel-Khalek; M. A. El-Sherbieny; Azza A. Helmy. "Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen". EKB Journal Management System, 12, 1, 2021, 47-54. doi: 10.21608/jappmu.2021.149460
Younan,, G., Shehab El-Din, A., Abdel-Khalek, A., El-Sherbieny, M., Helmy, A. (2021). 'Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen', EKB Journal Management System, 12(1), pp. 47-54. doi: 10.21608/jappmu.2021.149460
Younan,, G., Shehab El-Din, A., Abdel-Khalek, A., El-Sherbieny, M., Helmy, A. Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen. EKB Journal Management System, 2021; 12(1): 47-54. doi: 10.21608/jappmu.2021.149460

Soybean Lecithin as an Alternative to Egg Yolk in Tris-Based Extender of Cryopreserved Apri Rabbit Semen

Journal of Animal and Poultry Production
Article 8, Volume 12, Issue 1, January 2021, Page 47-54  XML PDF (956.9 K)
Document Type: Original Article
DOI: 10.21608/jappmu.2021.149460
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Authors
G. E. Younan, email orcid 1; A. M. Shehab El-Din1; A. E. Abdel-Khalek2; M. A. El-Sherbieny1; Azza A. Helmy1
1Animal Production Research Institute, Agricultural Research Center, Egypt.
2Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt.
Abstract
The present study was conducted to demonstrate whether soy lecithin could be used as an alternative to egg yolk (EY) in tris-extender for cryopreservation of APRI rabbit semen. In tris-extender (E1), 18% EY was replaced by 1% (E2), 1.5% (E3), or 2% (E4) SBL (1:4). Semen was evaluated visually after cryopreservation stages and by CASA only post-thawing. Results showed that visual sperm motility, livability, abnormality, acrosome integrity, and membrane integrity were improved (P<0.05) by E3 after all cryopreservation stages. Slow, total progressive, and total motility were the highest (P<0.05), while non-motility and non-progressive motility were the lowest (P<0.05) with E3 compared with other extenders. In comparing with E1, E3 showed the best dynamic sperm parameters, in terms of the highest VCL, STR (P<0.05), VSL (P≥0.05), and the lowest (P<0.05) WOB. Prolificacy of rabbits showed the highest (6.68 kits/doe, P<0.05) by E3 compared with 4.96, 4.72, and 2.8 kits/doe for E1, E2, and E4, respectively. The correlation coefficient was the lowest (r=0.691, P<0.05) between acrosome integrity and litter size, and the highest (r= 0.921, P<0.001) between membrane integrity and prolificacy.  Progressive motility and slow motility correlated with pregnancy (r=0.811and r=0.778, P<0.01) and prolificacy (r=0.898, P<0.001 and 0.801, P<0.01). In conclusion, addition of soybean lecithin at a level of 1.5% as an alternative to 18% egg yolk in tris extender may has a beneficial impact on motility parameters of cryopreserved APRI-rabbit spermatozoa, and prolificacy of doe rabbits.
Keywords
Rabbit; semen; egg yolk; lecithin; sperm function; sperm dynamic
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